Gannot G, Bermudez D, Lillibridge D, Fox P C
Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health.
Cell Death Differ. 1998 Sep;5(9):743-50. doi: 10.1038/sj.cdd.4400414.
Sjögren's syndrome (SS) is an autoimmune exocrinopathy characterized by mononuclear cell infiltration and loss of parenchymal tissue in salivary and lacrimal glands. The mechanisms for these histologic alterations are not known. Apoptotic cell death, induced by the ligation of Fas (APO-1/CD95) with Fas ligand (FasL/CD95L) may be an explanation for the tissue damage seen in SS. Fas and FasL were detected in minor salivary glands from SS patients and healthy individuals using immunohistochemical methods. There was increased expression of both Fas and FasL in the patients. The ability of the Fas-FasL pathway to influence epithelial cell growth and survival was demonstrated in vitro using a human submandibular cell line. The presence of Fas receptor was demonstrated on the cells. Anti-Fas antibody triggered cell death. Cells were also grown in the presence of gamma-interferon (IFN-gamma). IFN-gamma induced an upregulation of Fas receptor expression and pre-treatment of cells with IFN-gamma led to enhanced anti-Fas mediated cell death.
干燥综合征（SS）是一种自身免疫性外分泌腺病，其特征为唾液腺和泪腺出现单核细胞浸润以及实质组织丧失。这些组织学改变的机制尚不清楚。由Fas（APO-1/CD95）与Fas配体（FasL/CD95L）结合所诱导的凋亡性细胞死亡可能是SS中所见组织损伤的一种解释。采用免疫组化方法在SS患者和健康个体的小唾液腺中检测Fas和FasL。患者中Fas和FasL的表达均增加。使用人下颌下细胞系在体外证明了Fas-FasL途径影响上皮细胞生长和存活的能力。在细胞上证明了Fas受体的存在。抗Fas抗体引发细胞死亡。细胞也在γ干扰素（IFN-γ）存在的情况下生长。IFN-γ诱导Fas受体表达上调，用IFN-γ预处理细胞导致抗Fas介导的细胞死亡增强。
