Junquera F, Saperas E, de Torres I, Vidal M T, Malagelada J R
Pathology Department, Hospital General Vall d'Hebron, Autonomous University of Barcelona, Spain.
Am J Gastroenterol. 1999 Apr;94(4):1070-6. doi: 10.1111/j.1572-0241.1999.01017.x.
Angiodysplasia of the colon is a distinct vascular abnormality characterized by focal accumulation of ectatic vessels in the mucosa and submucosa. To investigate whether angiogenesis contributes to the pathogenesis of human colonic angiodysplasia, we examined the expression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), and its endothelial cell receptors flt-1 and KDR.
Immunohistochemistry was performed in sections of specimens obtained from 18 patients with colonic angiodysplasia and from eight patients with colon cancer and its adjacent, histologically normal margins of resection. We used affinity-purified rabbit polyclonal antibodies and a streptoavidin-biotin peroxidase method.
We detected strong immunoreactivity for vascular endothelial growth factor, homogeneously distributed in the endothelial lining of blood vessels of all sizes in 16 (89%) specimens of colonic angiodysplasia and in seven (88%) patients with colon cancer. In contrast, very limited immunoreactivity was found in normal colon. Vascular staining for flt-1 was observed in eight (44%) and one (12.5%) of the colonic angiodysplasia or colon cancer specimens, respectively, but not in normal colon. Vascular immunoreactivity for basic fibroblast growth factor was observed in seven (39%) specimens from patients with colonic angiodysplasia, whereas either very limited or no immunostaining was found in sections from specimens of patients with colon cancer and its normal margins.
In human colonic angiodysplasia, increased expression of angiogenic factors is likely to play a pathogenic role.
结肠血管发育异常是一种独特的血管异常，其特征是黏膜和黏膜下层出现扩张血管的局灶性聚集。为了研究血管生成是否参与人类结肠血管发育异常的发病机制，我们检测了碱性成纤维细胞生长因子（bFGF）、血管内皮生长因子（VEGF）及其内皮细胞受体flt-1和KDR的表达。
对18例结肠血管发育异常患者以及8例结肠癌患者及其组织学正常的癌旁切缘标本进行免疫组织化学检测。我们使用亲和纯化的兔多克隆抗体和链霉亲和素-生物素过氧化物酶法。
我们检测到血管内皮生长因子有强免疫反应性，在16例（89%）结肠血管发育异常标本和7例（88%）结肠癌患者的各种大小血管的内皮衬里中均匀分布。相比之下，在正常结肠中发现的免疫反应性非常有限。分别在8例（44%）结肠血管发育异常标本和1例（12.5%）结肠癌标本中观察到flt-1的血管染色，但在正常结肠中未观察到。在7例（39%）结肠血管发育异常患者的标本中观察到碱性成纤维细胞生长因子的血管免疫反应性，而在结肠癌患者及其正常切缘标本的切片中，要么免疫染色非常有限，要么未发现免疫染色。
在人类结肠血管发育异常中，血管生成因子表达增加可能起致病作用。
