Davison S P, McCaffrey T V, Porter M N, Manders E
Division of Plastic Surgery, University of Pittsburgh, Pennsylvania 15261, USA.
Laryngoscope. 1999 Apr;109(4):631-5. doi: 10.1097/00005537-199904000-00021.
Recovery of injured peripheral nerves depends on a balance between Schwann cell regeneration and scar formation. Transforming growth factor-beta1 (TGF-beta1) has been implicated as a humoral stimulus in scar formation. The neutralization of TGF-beta1 has been beneficial in the reduction of fibrosis. This study was to identify the presence of TGF-beta1 in regenerating peripheral nerve and to measure motor nerve regeneration by the neutralization of TGF-beta1 in neural wounds.
A randomized study of rat sciatic nerve regeneration.
Sciatic nerve axotomy was performed, followed by serial immunohistochemical staining by anti-TGF-beta1 at 12 to 216 hours (n = 5). Two groups (n = 10) with sciatic axotomy and epineural repair were treated with a 7-day perineural administration of neutralizing antibody of TGF-beta1 or saline carrier via subcutaneous silicone infusion port. A control group (n = 10) without axotomy with anti-TGF-beta1 administration was established. At 12 weeks the compound muscle action potential amplitude (CMAP) and the muscle twitch strength generated by the gastrocnemiussoleus muscle complex were measured.
TGF-beta1 was qualitatively present with maximal concentration by 72 to 144 hours. CMAP amplitude in the anti-TGF-beta1/axotomy group was 49.6% of the control and the axotomy/saline group was 31% of the control. Muscle twitch strength was 74% and 46.5%, respectively. These differences were statistically significant, P = .05.
The presence of TGF-beta1 at regenerating nerve sites was confirmed. The benefit of neutralization of transforming growth factor on CMAP and muscle twitch strength was shown. These results suggest improved regeneration at nerve injury sites with neutralization of TGF-beta1.
受损周围神经的恢复取决于施万细胞再生与瘢痕形成之间的平衡。转化生长因子β1（TGF-β1）被认为是瘢痕形成中的一种体液刺激因素。中和TGF-β1有助于减少纤维化。本研究旨在确定再生周围神经中TGF-β1的存在情况，并通过中和神经伤口中的TGF-β1来测量运动神经的再生情况。
一项关于大鼠坐骨神经再生的随机研究。
进行坐骨神经切断术，然后在12至216小时对5只大鼠进行抗TGF-β1的系列免疫组织化学染色。两组（每组10只）进行坐骨神经切断术并进行神经外膜修复，通过皮下硅胶输注端口给予TGF-β1中和抗体或生理盐水载体进行为期7天的神经周围给药。设立一个未进行切断术但给予抗TGF-β1的对照组（每组10只）。在12周时，测量复合肌肉动作电位幅度（CMAP）以及腓肠肌-比目鱼肌复合体产生的肌肉抽搐强度。
TGF-β1在72至144小时时定性存在且浓度最高。抗TGF-β1/切断术组的CMAP幅度为对照组的49.6%，切断术/生理盐水组为对照组的31%。肌肉抽搐强度分别为74%和46.5%。这些差异具有统计学意义，P = 0.05。
证实了再生神经部位存在TGF-β1。显示了中和转化生长因子对CMAP和肌肉抽搐强度的益处。这些结果表明，中和TGF-β1可改善神经损伤部位的再生。
