Pei Yuan-yuan, Duan Shao-bin, Cai Wei-jun, Yi Xi-nan, Zeng Zhi-cheng, Zhang Jian-wei, Xu Yuan-zhong, Zou Qiong-yan, Wen Xiao-dan
Department of Human Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha 410013, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Aug;30(4):447-51.
To explore the effects of exogenous transforming growth factor-beta 1 (TGFbeta1) on peripheral nerve regeneration after the peripheral nerve injury and if TGFbeta1 regulates the expression of basic fibroblast growth factor (bFGF) in the anterior horn motoneurons of spinal cord during regeneration.
Forty-eight rats were crushed on the right sciatic nerve and then randomly divided into 2 groups: TGFbeta1 group and NS group. In TGFbeta1 group, TGFbeta1 50 microL (0.1 microg/mL) was injected into the proximal nerve near to the crushed nerve and after the operation the injured leg was injected with equal TGFbeta1 whereas the NS was replaced in the NS group. The rats of each group survived for 3, 7, 14 and 21 days after the lesion. The bFGF expression in the anterior horn motoneurons of spinal cord was detected by immunohistochemistry (IHC). Semi-thin section and Fast Blue retrograde tracing were also performed with the rats surviving for 21 days to observe the regeneration of distal end in the injured right sciatic nerve.
The number of bFGF immunoreactive positive motoneurons in TGFbeta1 group was obviously higher than that of the NS group (P < 0.05). In the distal sciatic nerve of the rats treated with TGFbeta1, the number and diameter of regenerating myelinated axons and the thickness of myelinated sheath were more than those of the NS group (P < 0.05). The number of motoneurons in spinal cord and neurons in dorsol root ganglia (DRG) labelled with Fast Blue in the NS group was obviously lower than in the TGFbeta1 group (P < 0.01).
Exogenous TGFbeta1 plays an important role in promoting the peripheral nerve regeneration; TGFbeta1 up-regulates the bFGF expression in the anterior horn motoneurons of spinal cord during the peripheral nerve regeneration.
探讨外源性转化生长因子β1（TGFβ1）对周围神经损伤后周围神经再生的影响，以及TGFβ1在再生过程中是否调节脊髓前角运动神经元中碱性成纤维细胞生长因子（bFGF）的表达。
48只大鼠右侧坐骨神经被挤压后随机分为2组：TGFβ1组和生理盐水组。TGFβ1组于靠近挤压神经处的近端神经注射50 μL TGFβ1（0.1 μg/mL），术后于伤侧腿注射等量TGFβ1，生理盐水组则注射等量生理盐水。每组大鼠在损伤后3、7、14和21天存活。采用免疫组织化学（IHC）检测脊髓前角运动神经元中bFGF的表达。对存活21天的大鼠进行半薄切片和快蓝逆行追踪，观察右侧损伤坐骨神经远端的再生情况。
TGFβ1组bFGF免疫反应阳性运动神经元数量明显高于生理盐水组（P < 0.05）。在接受TGFβ1治疗的大鼠坐骨神经远端，再生有髓轴突的数量和直径以及髓鞘厚度均多于生理盐水组（P < 0.05）。生理盐水组中用快蓝标记的脊髓运动神经元数量和背根神经节（DRG）神经元数量明显低于TGFβ1组（P < 0.01）。
外源性TGFβ1在促进周围神经再生中起重要作用；TGFβ1在周围神经再生过程中上调脊髓前角运动神经元中bFGF的表达。
