2'-C-α-二氟甲基阿糖腺苷的合成及其3'-O-亚磷酰胺酯掺入锤头状核酶的研究

Synthesis of 2'-C-alpha-difluoromethylarauridine and its 3'-O-phosphoramidite incorporation into a hammerhead ribozyme.

作者信息

Dunkel M, Reither V

机构信息

Innovir GmbH, Rosdorf, Germany.

出版信息

Bioorg Med Chem Lett. 1999 Mar 8;9(5):787-92. doi: 10.1016/s0960-894x(99)00063-3.

DOI:10.1016/s0960-894x(99)00063-3

PMID:10201848

Abstract

The 2'-C-difluoromethylated nucleoside 4 was synthesized starting from uridine. 4 was then converted to the 3'-O-phosphoramidite derivative 5 and was incorporated into a hammerhead ribozyme (7). The cleavage characteristics of the modified oligonucleotide have been analysed.

摘要

2'-C-二氟甲基化核苷4是从尿苷开始合成的。然后将4转化为3'-O-亚磷酰胺衍生物5，并将其掺入锤头状核酶（7）中。已对修饰寡核苷酸的切割特性进行了分析。