Potter A, Kim C, Gollahon K A, Rabinovitch P S
Department of Pathology, University of Washington, Seattle, Washington, 98195, USA.
Cell Immunol. 1999 Apr 10;193(1):36-47. doi: 10.1006/cimm.1998.1443.
We used quantitative multiparameter flow cytometric assays to simultaneously detect viable, apoptotic, and necrotic human peripheral blood mononuclear cells (PBMC) and immunophenotyped lymphocyte subsets within the PBMC. Apoptosis was induced by a spectrum of treatments, including camptothecin, cisplatin, dexamethasone, hyperthermia, staurosporine, and etoposide in anti-CD3 mAb-stimulated cells and by cyclohexamide in both quiescent and stimulated cells; apoptosis in the latter was augmented by anti-fas mAb. We found that CD4(+) and CD8(+) cells were significantly underrepresented in the apoptotic PBMC and that the percentage of CD4(+) and CD8(+) PBMC each markedly decreased as apoptosis increased. This suggested that surface expression of these receptors was lessened on apoptotic CD4(+) and CD8(+) cells. This was directly confirmed by observation of sorted CD4(+) PBMC. This analysis of a wide variety of apoptotic stimuli demonstrates that diminished CD4 and CD8 surface receptor expression is a common feature of human T lymphocyte apoptosis.
我们采用定量多参数流式细胞术检测方法，同时检测存活的、凋亡的和坏死的人外周血单个核细胞（PBMC）以及PBMC内淋巴细胞亚群的免疫表型。凋亡通过一系列处理诱导产生，包括在抗CD3单克隆抗体刺激的细胞中使用喜树碱、顺铂、地塞米松、热疗、星形孢菌素和依托泊苷，以及在静止和刺激细胞中使用环己酰亚胺；后者中的凋亡通过抗Fas单克隆抗体增强。我们发现，在凋亡的PBMC中CD4(+)和CD8(+)细胞明显减少，并且随着凋亡增加，CD4(+)和CD8(+) PBMC的百分比均显著下降。这表明这些受体在凋亡的CD4(+)和CD8(+)细胞上的表面表达减少。通过对分选的CD4(+) PBMC的观察直接证实了这一点。对多种凋亡刺激的分析表明，CD4和CD8表面受体表达减少是人类T淋巴细胞凋亡的一个共同特征。
