用铜绿假单胞菌V抗原进行主动和被动免疫可预防III型中毒和肺损伤。

Active and passive immunization with the Pseudomonas V antigen protects against type III intoxication and lung injury.

作者信息

Sawa T, Yahr T L, Ohara M, Kurahashi K, Gropper M A, Wiener-Kronish J P, Frank D W

机构信息

Department of Anesthesia and Perioperative Care, The University of California, San Francisco 94143-0542, USA.

出版信息

Nat Med. 1999 Apr;5(4):392-8. doi: 10.1038/7391.

DOI:10.1038/7391

PMID:10202927

Abstract

Pseudomonas aeruginosa is an opportunistic bacterial pathogen that can cause fatal acute lung infections in critically ill individuals. Damage to the lung epithelium is associated with the expression of toxins that are directly injected into eukaryotic cells through a type Ill-mediated secretion and translocation mechanism. Here we show that the P. aeruginosa homolog of the Yersinia V antigen, PcrV, is involved in the translocation of type III toxins. Vaccination against PcrV ensured the survival of challenged mice and decreased lung inflammation and injury. Antibodies to PcrV inhibited the translocation of type III toxins.

摘要

铜绿假单胞菌是一种机会性细菌病原体，可在重症患者中引起致命的急性肺部感染。肺上皮损伤与通过III型介导的分泌和转运机制直接注入真核细胞的毒素表达有关。在这里，我们表明耶尔森氏菌V抗原的铜绿假单胞菌同源物PcrV参与III型毒素的转运。针对PcrV的疫苗接种确保了受攻击小鼠的存活，并减少了肺部炎症和损伤。抗PcrV抗体抑制III型毒素的转运。