单脐动脉脐带的体外灌注研究：血清素的血管活性作用。

In vitro perfusion studies of single umbilical artery cords: the vasoactive effects of serotonin.

作者信息

Haugen G, Moe N

机构信息

Department of Obstetrics and Gynecology, The National Hospital, University of Oslo, Norway.

出版信息

Acta Obstet Gynecol Scand. 1999 Apr;78(4):285-9.

PMID:10203293

Abstract

BACKGROUND

To investigate the vascular resistance and the vasoactive effects of serotonin in arteries from single umbilical artery (SUA) cords.

METHODS

The preparations were sampled from 10 patients with SUA cords. The control group (n=20) was matched according to gestational age, birth weight percentile, blood pressure and proteinuria. The arterial segments were perfused in vitro employing a constant perfusion rate and measuring alterations in perfusion pressure. Following an initial test with serotonin 10(-7) M the drug was administrated in stepwise increasing concentrations from 10(-10) to 10(-5) M.

RESULTS

The vascular resistance was significantly reduced in the SUA group compared to the control group (p<0.01). In about half of the preparations in both groups serotonin 10(-7) M induced a transient pressure decrease followed by a larger pressure increase. The remaining arteries showed a monophasic pressure increase. The maximum constrictory response calculated in percent of the prestimulatory perfusion pressure was significantly increased (p<0.01) in the SUA compared to the control group, but non-significantly increased (p=0.073) when calculated in absolute values (mmHg).

CONCLUSIONS

Arteries in SUA cords display reduced vascular resistance compared to arteries from normal cords in in vitro perfused preparations. The response pattern to serotonin was not altered, but the constrictory response might be increased in arteries from SUA cords.

摘要

背景

研究单脐动脉（SUA）脐带动脉中血清素的血管阻力及血管活性作用。

方法

从10例SUA脐带患者中采集样本。对照组（n = 20）根据孕周、出生体重百分位数、血压和蛋白尿进行匹配。采用恒定灌注速率对动脉段进行体外灌注，并测量灌注压力的变化。在初始使用10⁻⁷ M血清素测试后，以10⁻¹⁰至10⁻⁵ M的浓度逐步增加给药。

结果

与对照组相比，SUA组的血管阻力显著降低（p < 0.01）。在两组约一半的样本中，10⁻⁷ M血清素引起短暂的压力下降，随后是更大的压力升高。其余动脉显示单相压力升高。与对照组相比，SUA组以刺激前灌注压力百分比计算的最大收缩反应显著增加（p < 0.01），但以绝对值（mmHg）计算时无显著增加（p = 0.073）。