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通过酿酒酵母（delta）gcr3菌株的互补作用从白色念珠菌中分离GCR3同源物并进行测序。

Isolation and sequence of the GCR3 homologue from Candida albicans by complementation of (delta)gcr3 strain of Saccharomyces cerevisiae.

作者信息

Uemura H, Nakamoto K, Sugioka S, Tadenuma M

机构信息

Department of Molecular Biology, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki, Japan.

出版信息

Yeast. 1999 Mar 15;15(4):323-7. doi: 10.1002/(SICI)1097-0061(19990315)15:4<323::AID-YEA363>3.0.CO;2-L.

DOI:10.1002/(SICI)1097-0061(19990315)15:4<323::AID-YEA363>3.0.CO;2-L
PMID:10206191
Abstract

To study the function of GCR3, a gene involved in the expression of glycolytic genes in Saccharomyces cerevisiae, a Candida albicans gene which complements the growth defect of the (delta)gcr3 mutant was isolated. Transformants of this gene also recovered the glycolytic enzyme activities. Its DNA sequencing predicted an 886 amino acid protein with 30.4% identity to the Gcr3p of S. cerevisiae.

摘要

为研究参与酿酒酵母糖酵解基因表达的基因GCR3的功能，分离出了一个可弥补（δ）gcr3突变体生长缺陷的白色念珠菌基因。该基因的转化体也恢复了糖酵解酶活性。其DNA测序预测有一个886个氨基酸的蛋白质，与酿酒酵母的Gcr3p有30.4%的同源性。

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