Leroy M J, Méhats C, Duc-Goiran P, Tanguy G, Robert B, Dallot E, Mignot T M, Grangé G, Ferré F
Institut National de la Santé et de la Recherche Médicale, Unité U361, Maternité Port-Royal-Cochin, Université René Descartes, Paris, France.
Cell Signal. 1999 Jan;11(1):31-7. doi: 10.1016/s0898-6568(98)00028-x.
In light of the important role of the second messengers cAMP and cGMP in the mechanism of relaxation in the human myometrium, specific regulation of the phosphodiesterase (PDE) enzymatic system responsible for cyclic nucleotide inactivation is essential. We previously identified in the human myometrium PDE4 cAMP-specific PDE as by far the most abundant isoform. Here we have studied the expression patterns of mRNAs for the four cloned human PDE4 genes in the myometria of pregnant and non-pregnant women. Concurrent expression of the PDE4A, 4B, 4C and 4D genes is demonstrated. We found that the PDE4D transcripts are the most prominently expressed. PDE4A and PDE4B mRNAs also are markedly abundant, whereas lower expression is observed for PDE4C mRNAs. Interestingly, we showed that transcripts of PDE4B2 are more abundant in the myometria of pregnant women than in non-pregnant women, whereas no difference between the two tissues was detected for PDE4A, 4C and 4D mRNAs. Cultured human myometrial cells, which present a high level of PDE4 activity and express the four PDE4 mRNA subtypes, provide us with an appropriate model to further evaluate whether the level of expression of the PDE 4B2 mRNA subtype is under hormonal regulation.
鉴于第二信使环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）在人类子宫肌层舒张机制中发挥的重要作用，对负责环核苷酸失活的磷酸二酯酶（PDE）酶系统进行特异性调节至关重要。我们之前在人类子宫肌层中鉴定出，PDE4（cAMP特异性PDE）是迄今为止最丰富的亚型。在此，我们研究了4种克隆的人类PDE4基因的mRNA在孕妇和非孕妇子宫肌层中的表达模式。结果表明，PDE4A、4B、4C和4D基因同时表达。我们发现，PDE4D转录本表达最为显著。PDE4A和PDE4B的mRNA也明显丰富，而PDE4C的mRNA表达较低。有趣的是，我们发现PDE4B2的转录本在孕妇子宫肌层中比在非孕妇子宫肌层中更丰富，而PDE4A、4C和4D的mRNA在这两种组织中未检测到差异。培养的人类子宫肌层细胞具有高水平的PDE4活性并表达4种PDE4 mRNA亚型，这为我们提供了一个合适的模型，以进一步评估PDE 4B2 mRNA亚型的表达水平是否受激素调节。
