Jiang P S, Yung B Y
Cancer Biochemistry Laboratory, Department of Pharmacology, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-San, Tao-Yuan 333, Taiwan, Republic of China.
Biochem Biophys Res Commun. 1999 Apr 21;257(3):865-70. doi: 10.1006/bbrc.1999.0551.
In investigating the regulation of nucleophosmin/B23 mRNA expression at the entry of mitosis, the results of Northern gel blot analysis showed that the nucleophosmin/B23 mRNA levels significantly increased in prometaphase (nocodazole-arrested) or metaphase (colchicine-arrested) cells collected by mitotic shake-off. A higher level of nucleophosmin/B23 mRNA was detected in all the collected mitotic cells arrested by treatment with nocodazole for 10-18h as compared to that in G2 cells. An attempt was then made to determine whether the regulation of nucleophosmin/B23 mRNA plays a role in the control of entry into mitosis. Down-regulation of nucleophosmin/B23 mRNA by transfection of its antisense construct resulted in the delay of cells entering mitosis. The demonstration of the characteristic changes in the mRNA level of nucleophosmin/B23 during the entry of cells into mitosis implicates the importance of nucleophosmin/B23 in the control of the mitotic fate of nucleoli and cell growth.
在研究有丝分裂进入期核磷蛋白/B23 mRNA表达的调控时，Northern凝胶印迹分析结果显示，通过有丝分裂振荡收集的前中期（诺考达唑阻滞）或中期（秋水仙碱阻滞）细胞中，核磷蛋白/B23 mRNA水平显著升高。与G2期细胞相比，在用诺考达唑处理10 - 18小时阻滞的所有收集到的有丝分裂细胞中，检测到更高水平的核磷蛋白/B23 mRNA。随后尝试确定核磷蛋白/B23 mRNA的调控是否在有丝分裂进入的控制中起作用。通过转染其反义构建体下调核磷蛋白/B23 mRNA导致细胞进入有丝分裂延迟。细胞进入有丝分裂期间核磷蛋白/B23 mRNA水平的特征性变化表明，核磷蛋白/B23在控制核仁的有丝分裂命运和细胞生长中具有重要作用。
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