在星形孢菌素诱导的染色体解聚过程中，有丝分裂特异性形式的核磷蛋白/B23的积累和去磷酸化减少。
Decreased accumulation and dephosphorylation of the mitosis-specific form of nucleophosmin/B23 in staurosporine-induced chromosome decondensation.
作者信息
Lu Y Y, Lam C Y, Yung B Y
机构信息
Department of Pharmacology, Chang Gung Medical & Engineering College, Tao-Yuan, Taiwan, Republic of China.
出版信息
Biochem J. 1996 Jul 1;317 ( Pt 1)(Pt 1):321-7. doi: 10.1042/bj3170321.
Nucleophosmin/B23 is highly phosphorylated by cdc2 kinase during mitosis, and this phosphorylation most probably has a role in initiating and controlling the entry of cells into mitosis [Peter, Nakagawa, Doree, Labbe and Nigg (1990) Cell 60, 791-801]. In the present study, the protein kinase inhibitor staurosporine has been used to examine possible changes in nucleophosmin/B23 at mitosis in HeLa cells. Addition of staurosporine to HeLa cells already arrested at mitosis by nocodazole causes: (i) decreased accumulation of the mitosis-specific form of nucleophosmin/B23, (ii) dephosphorylation of nucleophosmin/ B23, (iii) redistribution of nucleophosmin/B23 to the cytosol, and (iv) concomitant decondensation of chromosomes. These results suggest that the mitosis-specific phosphorylated form of nucleophosmin/B23 may play a role in maintaining mitotic chromosomes in their condensed state.
核仁磷酸蛋白/B23在有丝分裂期间被cdc2激酶高度磷酸化，这种磷酸化很可能在启动和控制细胞进入有丝分裂过程中发挥作用[彼得、中川、多雷、拉贝和尼格（1990年）《细胞》第60卷，第791 - 801页]。在本研究中，蛋白激酶抑制剂星形孢菌素已被用于检测HeLa细胞在有丝分裂时核仁磷酸蛋白/B23可能发生的变化。将星形孢菌素添加到已被诺考达唑阻滞在有丝分裂期的HeLa细胞中会导致：（i）有丝分裂特异性形式的核仁磷酸蛋白/B23积累减少，（ii）核仁磷酸蛋白/B23去磷酸化，（iii）核仁磷酸蛋白/B23重新分布到细胞质中，以及（iv）同时染色体解聚。这些结果表明，有丝分裂特异性磷酸化形式的核仁磷酸蛋白/B23可能在维持有丝分裂染色体的凝聚状态中发挥作用。
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