Di Lorenzo G, Drago A, Pellitteri M E, Candore G, Colombo A, Potestio M, Di Salvo A, Mansueto S, Caruso C
Istituto di Medicina Interna e Geriatria, Università degli Studi di Palermo, Italy.
Allergy Asthma Proc. 1999 Mar-Apr;20(2):119-25. doi: 10.2500/108854199778612590.
The diagnostic value for allergies of the low affinity IgE receptor and its soluble circulating fragment (sCD23) remains unclear. In particular, little is know about seasonal influences on serum sCD23 levels in subjects with pollen allergy. In the present study, to gain insight into pathophysiological role of sCD23, we have analyzed, in blood from patients allergic to Parietaria sCD23, IgE, and eosinophil cationic protein (ECP) serum levels. IgE were assessed as atopy markers and ECP as an inflammation marker. Patients were studied during and out of pollen season, and results were compared to those obtained in nonallergic subjects. The study population included 42 nonsmoking outpatients, living in Palermo (Sicily, Italy) or in other west Sicilian towns, with a clinical diagnosis of seasonal asthma or rhinitis and monopositive skin test to Parietaria pollen. The group of asthmatic subjects consisted of 25 patients who had one or more of the usual asthma symptoms (wheezing, dyspnea, and cough) only during the pollen season. The group of rhinitis patients consisted of 17 patients, who, during pollen season, had the nasal symptoms (nasal blockage, sneezing, nasal itching, and rhinorrhoea) but no signs of asthma. As a control group, we studied 10 nonatopic subjects from laboratory staff. They had no history of seasonal or perennial rhinitis, asthma, or urticaria and had negative skin tests to a panel of allergens. Soluble CD23, IgE, and ECP were assessed in blood during and out of pollen season. Total serum IgE levels were clearly higher in atopic patients, as classically established. Concerning sCD23 serum levels, a similar pattern of results was obtained. Accordingly, significant correlations were shown between the levels of sCD23 and IgE in all groups of patients. A completely different pattern was observed by analyzing serum ECP levels because ECP levels were significantly increased only in asthmatic patients during pollen season. Accordingly, no significant correlations were observed between the levels of sCD23 and those of ECP. Identifying immune factors associated with the development of atopy can enhance our understanding of the in vivo mechanisms involved and may have utility in paradigms designed to prevent diseases. As demonstrated by the close correlation with total serum IgE values and the lack of correlation with serum ECP values, serum levels of sCD23 appear to be an additional marker for the diagnosis of atopy but not for the follow-up of allergic diseases.
低亲和力IgE受体及其可溶性循环片段（sCD23）对过敏症的诊断价值仍不明确。尤其是，对于花粉过敏患者血清sCD23水平的季节性影响知之甚少。在本研究中，为深入了解sCD23的病理生理作用，我们分析了对墙草属过敏患者血液中的sCD23、IgE和嗜酸性粒细胞阳离子蛋白（ECP）血清水平。IgE被评估为特应性标志物，ECP被评估为炎症标志物。在花粉季节期间和非花粉季节对患者进行研究，并将结果与非过敏受试者的结果进行比较。研究人群包括42名非吸烟门诊患者，他们居住在巴勒莫（意大利西西里岛）或西西里岛西部的其他城镇，临床诊断为季节性哮喘或鼻炎，且对墙草属花粉皮肤试验呈单阳性。哮喘组由25名患者组成，他们仅在花粉季节出现一种或多种常见的哮喘症状（喘息、呼吸困难和咳嗽）。鼻炎组由17名患者组成，他们在花粉季节出现鼻部症状（鼻塞、打喷嚏、鼻痒和流涕），但无哮喘迹象。作为对照组，我们研究了10名实验室工作人员中的非特应性受试者。他们没有季节性或常年性鼻炎、哮喘或荨麻疹病史，且对一组过敏原的皮肤试验呈阴性。在花粉季节期间和非花粉季节对血液中的可溶性CD23、IgE和ECP进行评估。如经典研究所示，特应性患者的总血清IgE水平明显更高。关于sCD23血清水平，获得了类似的结果模式。因此，在所有患者组中，sCD23水平与IgE水平之间均显示出显著相关性。通过分析血清ECP水平观察到完全不同的模式，因为仅在花粉季节哮喘患者中ECP水平显著升高。因此，未观察到sCD23水平与ECP水平之间存在显著相关性。识别与特应性发展相关的免疫因素可以增强我们对体内相关机制的理解，并且可能在旨在预防疾病的范例中具有实用价值。正如与总血清IgE值密切相关以及与血清ECP值缺乏相关性所表明的那样，血清sCD23水平似乎是诊断特应性的一个额外标志物，但不是用于过敏性疾病的随访。
