Türeci O, Sahin U, Zwick C, Neumann F, Pfreundschuh M
Department of Internal Medicine, University of Saarland, Homburg-Saar, Germany.
Hybridoma. 1999 Feb;18(1):23-8. doi: 10.1089/hyb.1999.18.23.
The screening of tumor-derived expression libraries for antigens which are recognized by high titered IgG antibodies present in autologous sera of the cancer patients by SEREX (serological identification of antigens by recombinant expression cloning) allows for the systematic identification of antigens in human cancers. SEREX has led to the definition of a plentitude of new tumor antigens in many different tumor entities. The majority of the antigens are encoded by hitherto unknown genes and can be grouped into different classes of antigens. The abundance of serologically defined human tumor antigens is not only of relevance for tumor biology and serodiagnosis of cancer, but also facilitates the identification of proteins recognized by tumor specific T lymphocytes, thus providing a molecular basis for polyvalent peptide-based and gene-therapeutic vaccine strategies in a wide variety of human neoplasms.
通过SEREX（重组表达克隆进行抗原的血清学鉴定）筛选肿瘤衍生表达文库，以寻找癌症患者自体血清中存在的高滴度IgG抗体所识别的抗原，这使得能够系统地鉴定人类癌症中的抗原。SEREX已在许多不同的肿瘤实体中定义了大量新的肿瘤抗原。大多数抗原由迄今未知的基因编码，并可分为不同类别的抗原。血清学定义的人类肿瘤抗原的丰富性不仅与肿瘤生物学和癌症的血清诊断相关，还有助于鉴定肿瘤特异性T淋巴细胞识别的蛋白质，从而为多种人类肿瘤中基于多价肽和基因治疗的疫苗策略提供分子基础。
