Wall R J, Foote R H
Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.
J Dairy Sci. 1999 Apr;82(4):817-21. doi: 10.3168/jds.S0022-0302(99)75301-4.
Egg yolk-Tris-glycerol extender, which is widely used in commercial artificial insemination (AI), was modified by replacing the 20% egg yolk (vol/vol) with a supernatant from egg yolk centrifuged at 50,000 x g for 2 h and then used to cryopreserve bull sperm. Preliminary studies showed that 20% egg yolk interfered with biochemical assays, which could be overcome by centrifugation. In Experiment 1, semen from 4 Holstein bulls was frozen in the experimental Tris extender and compared with the whole milk-glycerol control. A total of 2256 first services resulted in 72.6% 60- to 90-d nonreturns for the control and 71.0% for the Tris extender. In Experiment 2, semen from 10 Holstein bulls was frozen in the experimental Tris extender. Half of the semen was used immediately and half was stored in liquid nitrogen for 1 yr before distribution. The nonreturn rates based on 8878 first services for 7 bulls that completed both parts of the trial were 70.9% initially and 71.6% 1 yr later. This time trend difference of 0.7% was comparable to 0.6% for the AI mean for Holstein sires used at the same time. These fertility results and previous laboratory studies indicate that the conventional egg yolk-Tris-glycerol might be simplified for cryopreserving bull sperm. The experimental Tris extender also was suitable for making biochemical measurements.
蛋黄 - 三羟甲基氨基甲烷 - 甘油稀释液在商业人工授精（AI）中广泛使用，通过用50,000×g离心2小时的蛋黄上清液替代20%的蛋黄（体积/体积）对其进行了改良，然后用于冷冻保存公牛精液。初步研究表明，20%的蛋黄会干扰生化分析，而离心可以克服这一问题。在实验1中，将4头荷斯坦公牛的精液冷冻在实验性三羟甲基氨基甲烷稀释液中，并与全脂牛奶 - 甘油对照组进行比较。总共2256次首次输精，对照组60至90天的非返情率为72.6%，三羟甲基氨基甲烷稀释液组为71.0%。在实验2中，将10头荷斯坦公牛的精液冷冻在实验性三羟甲基氨基甲烷稀释液中。一半精液立即使用，另一半在液氮中储存1年后分发。基于7头完成试验两个部分的公牛的8878次首次输精，最初的非返情率为70.9%，1年后为71.6%。0.7%的时间趋势差异与同时使用的荷斯坦种公牛AI平均值的0.6%相当。这些生育力结果和先前的实验室研究表明，传统的蛋黄 - 三羟甲基氨基甲烷 - 甘油稀释液在冷冻保存公牛精液时可能可以简化。实验性三羟甲基氨基甲烷稀释液也适用于进行生化测量。
