Sarasquete C, Muñoz-Cueto J A, Ortiz J B, Rodríguez-Gómez F J, Dinis M T, Segner H
Institute of Sea Sciences of Andalucía, CSIC, Puerto Real, Cádiz, Spain.
Histol Histopathol. 1999 Apr;14(2):407-15. doi: 10.14670/HH-14.407.
CYP1A is a major inducible enzyme in the metabolism of xenobiotic substrates. In this paper we investigate by means of immunohistochemistry, the tissue distribution of constitutive cytochrome P4501A (CYP1A) during the period of endogenous nutrition (from hatching until day 4) in developing gilthead seabream, Sparus aurata larvae. For this purpose, a polyclonal antiserum (BN-1, Biosense Laboratories) directed against conserved piscine CYP1A sequences was used on paraffin-embedded sections from seabream larvae. From hatching onward, CYP1A immunoreactivity was observed in the following tissues and cells: syncytial, oil-globule envelopes and matrix of the yolk-sac, kidney (epithelia of renal tubules), cardiac muscle cells, skin epidermal cells, troncal musculature, enterocytes of different intestinal regions, goblet cells of the bucco-pharyngeal region, gill epithelial cells and the endothelia of the vascular system of various tissues (especially from liver and brain). Moreover, eye (retina), olfactory epithelium and some positive nerve fibers located in the proximity of the olfactory bulbs and running ventrally toward the posterior brain were strongly CYP1A immunoreactive. In general, the intensity of immunostaining increased with larval development.
细胞色素P450 1A（CYP1A）是外源生物底物代谢过程中的一种主要诱导酶。在本文中，我们通过免疫组织化学方法，研究了金头鲷（Sparus aurata）幼鱼在内源性营养期（从孵化到第4天）组成型细胞色素P450 1A（CYP1A）的组织分布。为此，我们使用了一种针对保守的鱼类CYP1A序列的多克隆抗血清（BN-1，Biosense Laboratories），对金头鲷幼鱼的石蜡包埋切片进行检测。从孵化开始，在以下组织和细胞中观察到CYP1A免疫反应性：合胞体、卵黄囊的油球包膜和基质、肾脏（肾小管上皮）、心肌细胞、皮肤表皮细胞、躯干肌肉组织、不同肠道区域的肠上皮细胞、口咽区域的杯状细胞、鳃上皮细胞以及各种组织（特别是肝脏和大脑）血管系统的内皮。此外，眼睛（视网膜）、嗅觉上皮以及位于嗅球附近并向后脑腹侧延伸的一些阳性神经纤维具有强烈的CYP1A免疫反应性。总体而言，免疫染色强度随着幼鱼发育而增加。
