Smith A, Robson L G, Sharma P, Shaw P J
Department of Cytogenetics, Royal Alexandra Hospital for Children, Westmead, New South Wales, Australia.
Pathology. 1999 Feb;31(1):25-8. doi: 10.1080/003130299105485.
We report here our experience in the management of four children undergoing sex mismatched allogeneic bone marrow transplantation (BMT) who showed evidence of poor engraftment post BMT. Fluorescence in situ hybridisation (FISH) was performed on direct bone marrow smears with the X centromere and Y heterochromatin probes, to ascertain chimerism. Of 16 hybridisations, only one was unsuccessful, whereas routine bone marrow cytogenetics performed at the same time failed in five of eight cultures. The FISH results were available in one to two days. The FISH findings provided a valuable aid in the early management of these children.
我们在此报告我们对四名接受性别不匹配异基因骨髓移植（BMT）的儿童的管理经验，这些儿童在BMT后显示出植入不良的证据。使用X着丝粒和Y异染色质探针在直接骨髓涂片上进行荧光原位杂交（FISH），以确定嵌合体情况。在16次杂交中，只有一次未成功，而同时进行的常规骨髓细胞遗传学在8次培养中有5次失败。FISH结果在1至2天内即可获得。FISH结果为这些儿童的早期管理提供了有价值的帮助。
