Ballestas M E, Chatis P A, Kaye K M
Department of Medicine, Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA.
Science. 1999 Apr 23;284(5414):641-4. doi: 10.1126/science.284.5414.641.
Primary effusion lymphoma (PEL) cells harbor Kaposi's sarcoma-associated herpesvirus (KSHV) episomes and express a KSHV-encoded latency-associated nuclear antigen (LANA). In PEL cells, LANA and KSHV DNA colocalized in dots in interphase nuclei and along mitotic chromosomes. In the absence of KSHV DNA, LANA was diffusely distributed in the nucleus or on mitotic chromosomes. In lymphoblasts, LANA was necessary and sufficient for the persistence of episomes containing a specific KSHV DNA fragment. Furthermore, LANA colocalized with the artificial KSHV DNA episomes in nuclei and along mitotic chromosomes. These results support a model in which LANA tethers KSHV DNA to chromosomes during mitosis to enable the efficient segregation of KSHV episomes to progeny cells.
原发性渗出性淋巴瘤（PEL）细胞含有卡波西肉瘤相关疱疹病毒（KSHV）附加体，并表达一种KSHV编码的潜伏相关核抗原（LANA）。在PEL细胞中，LANA和KSHV DNA在间期核内的点状物中以及沿有丝分裂染色体共定位。在没有KSHV DNA的情况下，LANA在核内或有丝分裂染色体上呈弥散分布。在淋巴母细胞中，LANA对于含有特定KSHV DNA片段的附加体的持续存在是必要且充分的。此外，LANA在核内以及沿有丝分裂染色体与人工KSHV DNA附加体共定位。这些结果支持了一种模型，即LANA在有丝分裂期间将KSHV DNA系附到染色体上，以使KSHV附加体能够有效地分离到子代细胞中。
