Adair B M, Cobb G P
Institute of Environmental and Human Health, Texas Tech University, Lubbock, USA.
Chemosphere. 1999 May;38(12):2951-8. doi: 10.1016/s0045-6535(98)00493-7.
Concentrations of mercury in biological samples collected for environmental studies are often less than 0.1 microgram/g. Low mercury concentrations and small organ sizes in many wildlife species (approximately 0.1 g) increase the difficulty of mercury determination at environmentally relevant concentrations. We have developed a digestion technique to extract mercury from small (0.1 g), biological samples at these relevant concentrations. Mean recoveries (+/- standard error) from validation trials of mercury fortified tissue samples using cold vapor atomic absorption spectroscopy for analysis ranged from 102 +/- 4.3% (2.5 micrograms/L, n = 15) to 108 +/- 1.4% (25 micrograms/L, n = 15). Recoveries of inorganic mercury were 99 +/- 5 (n = 19) for quality assurance samples analyzed during environmental evaluations conducted during a 24 month period. This technique can be used to determine total mercury concentrations of 60 ng Hg/g sample. Samples can be analyzed in standard laboratories in a short time, at minimal cost. The technique is versatile and can be used to determine mercury concentrations in several different matrices, limiting the time and expense of method development and validation.
在环境研究中收集的生物样本中的汞浓度通常低于0.1微克/克。许多野生动物物种（约0.1克）中汞浓度低且器官尺寸小，这增加了在环境相关浓度下测定汞的难度。我们开发了一种消化技术，用于从这些相关浓度下的小（0.1克）生物样本中提取汞。使用冷蒸气原子吸收光谱法进行分析的汞强化组织样本验证试验的平均回收率（±标准误差）范围为102±4.3%（2.5微克/升，n = 15）至108±1.4%（25微克/升，n = 15）。在为期24个月的环境评估期间分析的质量保证样本中，无机汞的回收率为99±5（n = 19）。该技术可用于测定每克样本60纳克汞的总汞浓度。样本可在标准实验室中短时间内以最低成本进行分析。该技术用途广泛，可用于测定几种不同基质中的汞浓度，从而限制了方法开发和验证的时间和费用。
