Eastwood C, Maubach K, Kirkup A J, Grundy D
Department of Biomedical Science, The University of Sheffield, Western Bank, UK.
Neurosci Lett. 1998 Oct 2;254(3):145-8. doi: 10.1016/s0304-3940(98)00666-1.
Some vagal afferent fibres are exquisitely sensitive to exogenous administration of cholecystokinin (CCK) but their sensitivity to endogenous CCK released by luminal stimuli has not been demonstrated directly, although implied from reflex and behavioural studies. We have therefore utilised electrophysiological techniques to record afferent discharge in mesenteric nerve bundles supplying the rat jejunum in response to luminal application of casein acid hydrolysate (CAH). CAH stimulated whole nerve afferent discharge in both in vivo and in vitro preparations (P < 0.01) while single unit analysis revealed that fibres sensitive to CAH also responded to exogenous CCK. The responses to both CCK and CAH were abolished by the CCKA antagonist devazepide. This study therefore supports the hypothesis that a functional relationship exists between CCK-containing enteroendocrine cells and the afferent fibres whose terminals lie within close proximity.
一些迷走传入纤维对外源性给予胆囊收缩素（CCK）极为敏感，尽管反射和行为学研究暗示了它们对腔内刺激释放的内源性CCK的敏感性，但尚未得到直接证实。因此，我们利用电生理学技术记录供应大鼠空肠的肠系膜神经束中的传入放电，以响应酪蛋白酸水解物（CAH）的腔内应用。CAH在体内和体外制剂中均刺激了全神经传入放电（P<0.01），而单单位分析显示，对CAH敏感的纤维对外源性CCK也有反应。CCKA拮抗剂德瓦西匹可消除对CCK和CAH的反应。因此，本研究支持这样一种假说，即含CCK的肠内分泌细胞与终末位于其附近的传入纤维之间存在功能关系。
