Ciruela F, Soloviev M M, McIlhinney R A
Medical Research Council Anatomical Neuropharmacology Unit, Oxford, UK.
FEBS Lett. 1999 Apr 1;448(1):91-4. doi: 10.1016/s0014-5793(99)00341-5.
The cell surface expression of metabotropic glutamate receptor type I splice variants has been studied using cell surface biotinylation. Co-expression of the last 86 residues of the C-terminal tail of mGluR1alpha (F2-protein) with mGluR1alpha caused a significant reduction of the amount of the cell surface receptor when compared to that in cells transfected with mGlur1alpha alone, and this was accompanied by a reduction in the production of inositol following agonist stimulation of the cells. In contrast, cell surface expression of mGluR1beta was unaltered by co-expression with the F2-protein. These results suggest that the C-terminal tail of mGluR1alpha regulates cell surface expression of the receptor.
