Hanley S Z, Pappin D J, Rahman D, White A J, Elborough K M, Slabas A R
Department of Biological Sciences, University of Durham, UK.
FEBS Lett. 1999 Mar 19;447(1):99-105. doi: 10.1016/s0014-5793(99)00235-5.
Sequence analysis of several cDNAs encoding the phasin protein of Ralstonia eutropha indicated that the carboxyl terminus of the resulting derived protein sequence is different from that reported previously. This was confirmed by: (1) sequencing of the genomic DNA; (2) SDS-PAGE and peptide analysis of wild-type and recombinant phasin; and (3) mass spectrometry of wild-type phasin protein. The results have implications for the model proposed for the binding of this protein to polyhydroxyalkanoic acid granules in the bacterium.
对几种编码真养产碱菌（Ralstonia eutropha）phasins蛋白的cDNA进行序列分析表明，所得推导蛋白序列的羧基末端与先前报道的不同。这通过以下方式得到证实：（1）基因组DNA测序；（2）野生型和重组phasins的SDS-PAGE和肽分析；以及（3）野生型phasins蛋白的质谱分析。这些结果对提出的关于该蛋白在细菌中与聚羟基脂肪酸酯颗粒结合的模型具有重要意义。
