羟酰基辅酶A脱水酶和反式-2,3-烯酰基辅酶A还原酶的活性与大鼠脑发育过程中长链脂肪酸的积累一致。

Hydroxyacyl-CoA dehydrase and trans-2,3-enoyl-CoA reductase activities are consistent with long-chain fatty acid accumulation during rat brain development.

作者信息

Knoll A, Sargueil F, Salles J, Garbay B, Lucet-Levannier K, Cassagne C

机构信息

Laboratoire de Biogenèse Membranaire, CNRS-UMR 5544, Université Victor Segalen Bordeaux 2, France.

出版信息

Neurosci Lett. 1999 Mar 19;263(1):5-8. doi: 10.1016/s0304-3940(99)00094-4.

DOI:10.1016/s0304-3940(99)00094-4

PMID:10218897

Abstract

The developmental changes of microsomal 3-hydroxyacyl-CoA dehydrase and trans-2,3-enoyl-CoA reductase activities were analyzed and compared to very-long-chain fatty acid content and biosynthesis in rat brain. Contrary to the elongation rate of eicosanoyl-CoA and 3-hydroxyeicosanoyl-CoA, which paralleled myelination during brain maturation, the two partial activities of fatty acid elongation were already present at the earliest stages of development. One day after birth, 3-hydroxyacyl-CoA dehydrase and trans-2,3-enoyl-CoA reductase specific activities already represented 54.8% and 49.6% of the adult values, respectively. As a contribution to the quantitative estimation of the brain's ability to form its own VLCFA, it is shown that dehydrase and reductase activities are sufficient to allow the biosynthesis of all rat brain VLCFA at any age.

摘要

分析了微粒体3-羟酰基辅酶A脱水酶和反式-2,3-烯酰基辅酶A还原酶活性的发育变化，并将其与大鼠脑中极长链脂肪酸含量和生物合成进行了比较。与花生四烯酰辅酶A和3-羟基花生四烯酰辅酶A的延长率相反，在脑成熟过程中，这两种脂肪酸延长率与髓鞘形成平行，而脂肪酸延长的两个部分活性在发育的最早阶段就已经存在。出生后一天，3-羟酰基辅酶A脱水酶和反式-2,3-烯酰基辅酶A还原酶的比活性分别已达到成年值的54.8%和49.6%。为了定量评估大脑形成自身极长链脂肪酸的能力，研究表明，脱水酶和还原酶活性足以在任何年龄进行所有大鼠脑极长链脂肪酸的生物合成。