Tamim S, Lauret M G, Drouet M, Sabbah A
Laboratoire d'Immuno-Allergologie-C.H.U., Angers, France.
Allerg Immunol (Paris). 1999 Feb;31(2):35-44.
Histamine Release technic consists in calculating histamine liberated by blood cells in touch with an allergen. To this day, this method is only used in Hymenoptera venom allergy diagnosis. The principle of this study is to measure histamine released by activated basophils in surnageons of Basophil Activation Test (BAT) for different allergens: Hymenoptera venoms: Bee, White Faced Hornet, Vespula Wasp. Drugs: Cefaperos, Clamoxyl, Alfatil, Rapifen, Diprivan, Nesdonal, Mivacron. A threshold of positivity (amplification factor in comparison with the control) is determined for these two classes of allergens: 45 for Hymenoptera venoms and 9 for drugs. These results, compared to the other diagnosis technics (Histamine Release, Basophil Activation Test, Prick Tests) discloses very high correlation rates in each case. This method seems to be a reliable method for Hymenoptera venom allergy diagnosis and for drugs allergy diagnosis too. However, this study is based on a few number of patients, so a significant statistic conclusion can't be expressed but it opens an interesting way of research.
组胺释放技术在于计算与变应原接触的血细胞释放的组胺量。时至今日，该方法仅用于膜翅目毒液过敏诊断。本研究的原理是在嗜碱性粒细胞活化试验（BAT）的上清液中测量不同变应原激活的嗜碱性粒细胞释放的组胺：膜翅目毒液：蜜蜂、白脸大黄蜂、胡蜂。药物：头孢哌酮、克拉霉素、阿法替尼、瑞芬太尼、丙泊酚、奈司他汀、米库氯铵。为这两类变应原确定阳性阈值（与对照相比的放大倍数）：膜翅目毒液为45，药物为9。这些结果与其他诊断技术（组胺释放、嗜碱性粒细胞活化试验、点刺试验）相比，在每种情况下都显示出非常高的相关性。该方法似乎是一种用于膜翅目毒液过敏诊断以及药物过敏诊断的可靠方法。然而，本研究基于少数患者，因此无法得出有统计学意义的结论，但它开辟了一条有趣的研究途径。
