Fieger-Büschges H, Schüssler G, Larsimont V, Blume H
Zentrallaboratorium Deutscher Apotheker, Eschborn, Germany.
J Chromatogr B Biomed Sci Appl. 1999 Mar 19;724(2):281-6. doi: 10.1016/s0378-4347(98)00530-1.
A rapid automated method has been developed for the determination of clindamycin, a lincosamide antibiotic, in human plasma. Coupled column HPLC was used after precipitation of plasma proteins with a saturated ammonium sulfate solution. As a first step, the drug and internal standard were trapped on a precolumn of LiChrospher 60RP-select B. A reversed-phase Nucleosil 100 C18 HD column then separated drug and internal standard from each other and from remaining plasma components. The assay was validated in the range 0.2-10.0 micrograms ml-1 plasma. The results obtained for accuracy, intra- and inter-day precision complied very well with the generally accepted criteria for bioanalytical assays.
已开发出一种快速自动化方法，用于测定人血浆中林可酰胺类抗生素克林霉素。在用饱和硫酸铵溶液沉淀血浆蛋白后，采用串联柱高效液相色谱法。第一步，药物和内标被捕获在LiChrospher 60RP-select B预柱上。然后，反相Nucleosil 100 C18 HD柱将药物和内标彼此分离，并与其余血浆成分分离。该测定法在0.2 - 10.0微克/毫升血浆范围内得到验证。所获得的准确度、日内和日间精密度结果与生物分析测定法普遍接受的标准非常吻合。
