23F型肺炎链球菌荚膜生物合成基因座的分子与遗传学特征分析

Molecular and genetic characterization of the capsule biosynthesis locus of Streptococcus pneumoniae type 23F.

作者信息

Morona Judy K, Miller David C, Coffey Tracey J, Vindurampulle Christofer J, Spratt Brian G, Morona Renato, Paton James C

机构信息

Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, South Australia 5006, Australia.

Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, Oxford OX1 3PS, UK.

出版信息

Microbiology (Reading). 1999 Apr;145 ( Pt 4):781-789. doi: 10.1099/13500872-145-4-781.

DOI:10.1099/13500872-145-4-781

PMID:10220157

Abstract

The authors have previously reported the nucleotide sequence of the 5' and 3' portions of the Streptococcus pneumoniae type 23F capsular polysaccharide biosynthesis locus (cps23f) (from dexB to cps23fB and from cps23fL to aliA). These regions of cps23f were very similar to the sequence reported for cps19f, the capsule locus of S. pneumoniae type 19F. However, Southern hybridization analysis indicated that no other genes closely related to cps19f are present in the cps23f locus. In this study long-range PCR was used to amplify and clone the section of the S. pneumoniae type 23F capsule locus between cps23fB and cps23fL. This region is 13 kb in size and contains 12 new ORFs, designated cps23fC-E, I, J, and T-Z. Functions are proposed for all of the protein products, including functional homologues of Cps19fC-E, Cps19fI and Cps19fJ. A biosynthetic pathway for type 23F capsular polysaccharide is also proposed.

摘要

作者之前曾报道过肺炎链球菌23F型荚膜多糖生物合成基因座（cps23f）（从dexB到cps23fB以及从cps23fL到aliA）5'和3'部分的核苷酸序列。cps23f的这些区域与已报道的肺炎链球菌19F型荚膜基因座cps19f的序列非常相似。然而，Southern杂交分析表明，cps23f基因座中不存在与cps19f密切相关的其他基因。在本研究中，使用长距离PCR扩增并克隆了肺炎链球菌23F型荚膜基因座中位于cps23fB和cps23fL之间的片段。该区域大小为13 kb，包含12个新的开放阅读框，命名为cps23fC - E、I、J以及T - Z。对所有蛋白质产物的功能进行了推测，包括Cps19fC - E、Cps19fI和Cps19fJ的功能同源物。还提出了23F型荚膜多糖的生物合成途径。