Lu Y T, Love N G, Grady C P
Department of Environmental Engineering and Sciences, Clemson University, SC 29634-0919, USA.
FEMS Microbiol Lett. 1999 Apr 1;173(1):195-201. doi: 10.1111/j.1574-6968.1999.tb13502.x.
Microscopic methods were developed that enable the sensitive quantification of different cell types that are generated by plasmid instability processes when Pseudomonas putida PaW164 (X+), which carries a TOL plasmid (pWW0-164), is grown in chemostat culture. Cells that have lost the structural TOL genes (X-) or the entire TOL plasmid (X0) can be quantified in a background of 6000 X+ cells using catechol agarose miniplates. X0 cells can be quantified in a background of 3500 X+ or X- cells using carbenicillin agarose miniplates. These methods represent significant improvements in sensitivity over conventional plating methods.
当携带TOL质粒（pWW0 - 164）的恶臭假单胞菌PaW164（X +）在恒化器培养中生长时，开发了一些微观方法，这些方法能够灵敏地定量由质粒不稳定过程产生的不同细胞类型。使用儿茶酚琼脂糖微孔板，可以在6000个X +细胞的背景下对失去结构TOL基因的细胞（X -）或整个TOL质粒的细胞（X0）进行定量。使用羧苄青霉素琼脂糖微孔板，可以在3500个X +或X -细胞的背景下对X0细胞进行定量。这些方法在灵敏度方面比传统的平板接种方法有显著提高。
