Szramka B, Kurlenda J, Bielawski K
Katedra Biotechnologii-Miedzyuczelniany Wydział Biotechnologii, Uniwersytetu Gdańskiego i Akademii Medycznej w Gdańsku.
Med Dosw Mikrobiol. 1998;50(3-4):207-13.
We demonstrated that Klebsiella pneumoniae and Klebsiella oxytoca possess a selective haemolytic activity on rabbit erythrocytes. Thirty one Klebsiella strains (18 strains of K. pneumoniae and 13 strains of K. oxytoca) were isolated from hospitalized patients. The liquid (Trypcase-soy broth--TSB) and solid (Trypcase-soy agar--TSA) medium, containing the red cells were used for the tests. All the screened strains showed a haemolytic effect on rabbit erythrocytes, provided that the supernatants of the cultures were preincubated with beta-mercaptoethanol or calcium chloride. There was no human and sheep erythrocyte lysis.
我们证明了肺炎克雷伯菌和产酸克雷伯菌对兔红细胞具有选择性溶血活性。从住院患者中分离出31株克雷伯菌(18株肺炎克雷伯菌和13株产酸克雷伯菌)。含有红细胞的液体(胰蛋白胨大豆肉汤 - TSB)和固体(胰蛋白胨大豆琼脂 - TSA)培养基用于测试。所有筛选出的菌株对兔红细胞均表现出溶血作用,前提是培养物的上清液先用β-巯基乙醇或氯化钙进行预孵育。未出现人红细胞和羊红细胞裂解现象。