Mitsui Y, Schmelzer J D, Zollman P J, Mitsui M, Tritschler H J, Low P A
Department of Neurology, Mayo Clinic, Rochester, MN 55905, USA.
J Neurol Sci. 1999 Feb 1;163(1):11-6. doi: 10.1016/s0022-510x(99)00017-9.
Reperfusion aggravates nerve ischemic fiber degeneration, likely by the generation of reduced oxygen species. We therefore evaluated if racemic alpha-lipoic acid (LA), a potent antioxidant, will protect peripheral nerve from reperfusion injury, using our established model of ischemia-reperfusion injury.
We used male SD rats, 300+/-5 g. Ischemia was produced by the ligature of each of the supplying arteries to the sciatic-tibial nerve of the right hind-limb for predetermined periods of time (either 3 or 5 h), followed by the release of the ligatures, resulting in reperfusion. LA was given intraperitoneally daily for 3 days for both pre- and post-surgery. Animals received either LA, 100 mg/kg/day, or the same volume of saline intraperitoneally. Clinical behavioral score and electrophysiology of motor and sensory nerves were obtained at 1 week after ischemia-reperfusion. After electrophysiological examination, the sciatic-tibial nerve was fixed in situ and embedded in epon. We evaluated for ischemic fiber degeneration (IFD) and edema, as we described previously.
Distal sensory conduction (amplitude of sensory action potential and sensory conduction velocity (SCV) of digital nerve) was significantly improved in the 3-h ischemia group, treated with LA (P<0.05). LA also improved IFD of the mid tibial nerve (P=0.0522). LA failed to show favorable effects if the duration of ischemia was longer (5-h ischemia).
These results suggest that alpha-lipoic acid is efficacious for moderate ischemia-reperfusion, especially on distal sensory nerves.
再灌注可能通过产生活性氧加重神经缺血性纤维变性。因此，我们使用已建立的缺血再灌注损伤模型，评估了消旋α-硫辛酸（LA）这种强效抗氧化剂是否能保护周围神经免受再灌注损伤。
我们使用体重300±5 g的雄性SD大鼠。通过结扎右后肢坐骨-胫神经的各供应动脉预定时间（3或5小时）造成缺血，随后松开结扎线，实现再灌注。术前和术后连续3天每天腹腔注射LA。动物腹腔注射100 mg/kg/天的LA或相同体积的生理盐水。缺血再灌注1周后进行临床行为评分以及运动和感觉神经的电生理检查。电生理检查后，将坐骨-胫神经原位固定并包埋在环氧树脂中。如我们之前所述，评估缺血性纤维变性（IFD）和水肿情况。
在3小时缺血组中，接受LA治疗的大鼠其远端感觉传导（指神经感觉动作电位幅度和感觉传导速度（SCV））显著改善（P<0.05）。LA还改善了胫神经中部的IFD（P=0.0522）。如果缺血时间更长（5小时缺血），LA则未显示出有益效果。
这些结果表明，α-硫辛酸对中度缺血再灌注有效，尤其是对远端感觉神经。
