Vickery K, Cossart Y, Dixon R
Department of Infectious Diseases, University of Sydney, NSW, Australia.
J Med Virol. 1999 May;58(1):19-25.
Duck hepatitis B virus (DHBV) has been a useful model for hepadnavirus infection. There have been few studies on immunity to DHBV and none describing the cell-mediated immune response by acute and chronically infected ducks. A duck hepatitis B antigen-specific blastogenesis assay was used to measure DHBV antigen-specific responses of duck peripheral blood (PBMC) and splenic mononuclear cells (SMCs) from uninfected control ducks, ducks acutely or chronically infected with DHBV, and ducks immune to DHBV. A comparison of the group mean responses by PBMC to DHBV surface antigen (DHBsAg) found that the immune group was significantly different to the other three groups (controls or unexposed, P < 0.0001; acutely infected, P< 0.01; chronically infected, P < 0.01). The responses to DHBsAg by PBMC of the acute group (P< 0.01) were significantly different also to that of the unexposed group. For DHBV core antigen (DHBcAg), significant differences in the responses were found between immune ducks and unexposed (P < 0.0005) and acutely infected (P < 0.05) groups. The SMC showed a significant difference between unexposed ducks and immune ducks (P< 0.05) in the group mean responses to DHBsAg. The responses to DHBcAg were significantly different between the immune group and the acute (P < 0.01) and unexposed (P < 0.01) groups. The group mean of unexposed ducks was also significantly different to that of acutely infected ducks (P < 0.01). This study indicates that the cellular immune response in immune animals differs from acutely and chronically infected ducks. Further studies of these differences may provide some explanations for the differing outcomes of DHBV infection.
鸭乙型肝炎病毒（DHBV）一直是研究嗜肝DNA病毒感染的有用模型。关于鸭对DHBV的免疫研究较少，且尚无研究描述急性和慢性感染鸭的细胞介导免疫反应。采用鸭乙型肝炎抗原特异性增殖试验，检测未感染对照鸭、急性或慢性感染DHBV的鸭以及对DHBV免疫的鸭外周血单个核细胞（PBMC）和脾单个核细胞（SMC）的DHBV抗原特异性反应。比较PBMC对DHBV表面抗原（DHBsAg）的组平均反应发现，免疫组与其他三组（对照组或未接触组，P<0.0001；急性感染组，P<0.01；慢性感染组，P<0.01）有显著差异。急性组PBMC对DHBsAg的反应（P<0.01）与未接触组也有显著差异。对于DHBV核心抗原（DHBcAg），免疫鸭与未接触组（P<0.0005）和急性感染组（P<0.05）的反应存在显著差异。SMC对DHBsAg的组平均反应在未接触鸭和免疫鸭之间存在显著差异（P<0.05）。免疫组与急性感染组（P<0.01）和未接触组（P<0.01）对DHBcAg的反应有显著差异。未接触鸭的组平均值与急性感染鸭的组平均值也有显著差异（P<0.01）。本研究表明，免疫动物的细胞免疫反应与急性和慢性感染鸭不同。对这些差异的进一步研究可能为DHBV感染的不同结果提供一些解释。
