5-脂氧合酶转录的遗传决定因素
Genetic determinants of 5-lipoxygenase transcription.
作者信息
Drazen J M, Silverman E S
机构信息
Division of Pulmonary and Critical Care Medicine, Departments of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, MA, USA.
出版信息
Int Arch Allergy Immunol. 1999 Feb-Apr;118(2-4):275-8. doi: 10.1159/000024098.
BACKGROUND
5 Lipoxygenase (5-LO) is a critical enzyme in the production of the leukotrienes. We have identified a series of mutations in the 5-LO gene that modify gene transcription. These mutations consist of addition of an Sp-1 binding motif (-GGGCGG-) or deletion of one or two Sp-1 binding motifs in the 5-LO core promoter.
METHODS
Mutant forms of the 5-LO core promoter were placed in a chloramphenicol acetyl transferase (CAT) reporter construct using either HeLa or SL-2 cells.
RESULTS
In HeLa cells all of the mutant forms are less effective in driving CAT reporter activity than the wild-type promoter. In SL-2 cells the construct containing the addition mutation was more effective in driving CAT reporter activity, while the constructs containing the deletion mutations were less effective.
CONCLUSIONS
These data indicate that naturally occurring mutations in the 5-LO core promoter modify gene transcription in vitro.
背景
5-脂氧合酶（5-LO）是白三烯生成过程中的关键酶。我们已经在5-LO基因中鉴定出一系列可改变基因转录的突变。这些突变包括在5-LO核心启动子中添加一个Sp-1结合基序（-GGGCGG-）或缺失一个或两个Sp-1结合基序。
方法
使用HeLa或SL-2细胞，将5-LO核心启动子的突变形式置于氯霉素乙酰转移酶（CAT）报告基因构建体中。
结果
在HeLa细胞中，所有突变形式驱动CAT报告基因活性的效果均不如野生型启动子。在SL-2细胞中，含有添加突变的构建体驱动CAT报告基因活性的效果更佳，而含有缺失突变的构建体效果则较差。
结论
这些数据表明，5-LO核心启动子中的自然发生突变在体外可改变基因转录。
Zotero 插件