Mutus B, Redmond R W, Akhter S
Chemistry and Biochemistry, School of Physical Sciences, University of Windsor, Ont., Canada.
FEBS Lett. 1999 Apr 16;449(1):79-82. doi: 10.1016/s0014-5793(99)00311-7.
Flash photolysis of S-nitrosoglutathione (GSNO) in aerated solutions at pH 10 gave rise to an absorption with a maximum around 310-320 nm. This peak is spectrally similar to that displayed by ONOO-. The decay kinetics of this absorption was compared to that of authentic ONOO-, generated independently. An excellent correlation was obtained. Further proof of ONOO- generation was provided by HPLC studies showing the production of 3-nitrotyrosine on irradiation of GSNO in the presence of tyrosine at pH 7.4. In addition, the nitration yield was increased approximately 5-fold in the presence of bicarbonate and totally eliminated with DMPO, indicating the requirement of a radical intermediate for peroxynitrite production during S-nitrosothiol photolysis.
在pH值为10的曝气溶液中对S-亚硝基谷胱甘肽（GSNO）进行闪光光解，产生了一个最大吸收峰在310 - 320纳米左右的吸收光谱。该峰在光谱上与过氧亚硝酸根（ONOO⁻）显示的峰相似。将该吸收光谱的衰减动力学与独立产生的纯ONOO⁻的衰减动力学进行了比较，得到了极好的相关性。通过HPLC研究进一步证明了ONOO⁻的产生，该研究表明在pH值为7.4的条件下，酪氨酸存在时照射GSNO会产生3-硝基酪氨酸。此外，在碳酸氢盐存在下硝化产率增加了约5倍，而在DMPO存在下则完全消除，这表明在S-亚硝基硫醇光解过程中过氧亚硝酸根产生需要自由基中间体。
