人26S蛋白酶体亚基11的鉴定、分子克隆及特性分析

Identification, molecular cloning, and characterization of subunit 11 of the human 26S proteasome.

作者信息

Hoffman L, Gorbea C, Rechsteiner M

机构信息

University of Utah School of Medicine, Department of Biochemistry, Salt Lake City 84132, USA.

出版信息

FEBS Lett. 1999 Apr 16;449(1):88-92. doi: 10.1016/s0014-5793(99)00403-2.

DOI:10.1016/s0014-5793(99)00403-2

PMID:10225435

Abstract

We sequenced five peptides from subunit 11 (S11), a 43 kDa protein of the human 26S proteasome, and used this information to clone its cDNA. The S11 cDNA encodes a 376 amino acid protein with a pI of 5.6 and a molecular mass of 42.9 kDa. Translation of S11 RNA in the presence of [35S]methionine produces a radiolabeled protein that co-migrates with S11 of the human 26S proteasome on SDS-PAGE. Polyclonal antiserum made against recombinant S11 recognizes a protein of the same size in extracts of bacteria expressing S11 and in purified 26S proteasomes from human red blood cells or rabbit reticulocytes. The S11 sequence does not contain motifs that suggest a biological function. S11 is, however, the human homolog of Rpn9, a recently identified subunit of the yeast 26S proteasome.

摘要

我们对人26S蛋白酶体中一个43 kDa的蛋白质——亚基11（S11）的五个肽段进行了测序，并利用这些信息克隆了其cDNA。S11 cDNA编码一个含376个氨基酸的蛋白质，其等电点为5.6，分子量为42.9 kDa。在[35S]甲硫氨酸存在的情况下，S11 RNA的翻译产生一种放射性标记的蛋白质，该蛋白质在SDS-PAGE上与人26S蛋白酶体的S11迁移率相同。针对重组S11制备的多克隆抗血清在表达S11的细菌提取物以及来自人红细胞或兔网织红细胞的纯化26S蛋白酶体中识别出大小相同的蛋白质。S11序列不包含提示生物学功能的基序。然而，S11是酵母26S蛋白酶体最近鉴定的亚基Rpn9的人类同源物。