Shapira L, Houri-Haddad Y, Frolov I, Halabi A, Ben-Nathan D
Department of Periodontology, Hebrew University - Hadassah School of Dental Medicine, Jerusalem, Israel.
J Periodontol. 1999 Mar;70(3):289-93. doi: 10.1902/jop.1999.70.3.289.
The impact of emotional stress on the outcome of infectious diseases was studied in animal models and humans, but data related to the effect of stress on periodontal infection are limited. Using the subcutaneous chamber model in mice, the present study was carried out to investigate the effect of stress on the host response to Porphyromonas gingivalis.
Mice with subcutaneous chambers (2 per animal) were divided into 4 treatment groups: cold-stress; isolation-stress; corticosterone (CS)-injected; and controls. On the third day of stress conditions, heat-killed P. gingivalis were injected into the chambers. The chambers were sampled 1 and 5 days later and analyzed for leukocyte number, tumor necrosis factor (TNF)-alpha levels, and interferon (IFN)-gamma levels.
Injection of P. gingivalis induced the migration of leukocytes into the chambers and increased the intrachamber levels of IFN-gamma and TNF-alpha. There were no significant differences in cell number and IFN-gamma levels between the different treatment groups, but the levels of TNF-alpha were significantly lower in the isolation-stress and cold-stress groups compared to control animals. CS-injected animals were not different from controls. In addition, the levels of TNF-alpha in the stressed animals were lower on the fifth day post-injection than on the first day, but not in the CS and control group.
The results suggest that the levels of TNF-alpha induced by P. gingivalis in the infection site are downregulated in stressed animals, and CS is not the sole mediator responsible. The stress-induced reduction in TNF-alpha levels might have an impact on the pathogenesis of periodontal disease in humans experiencing emotional stress.
在动物模型和人类中研究了情绪应激对传染病结局的影响，但与应激对牙周感染影响相关的数据有限。本研究利用小鼠皮下腔室模型，旨在探讨应激对宿主针对牙龈卟啉单胞菌反应的影响。
将带有皮下腔室的小鼠（每只动物2个）分为4个治疗组：冷应激组；隔离应激组；注射皮质酮（CS）组；以及对照组。在应激条件的第三天，将热灭活的牙龈卟啉单胞菌注入腔室。1天和5天后对腔室进行采样，并分析白细胞数量、肿瘤坏死因子（TNF）-α水平和干扰素（IFN）-γ水平。
注射牙龈卟啉单胞菌可诱导白细胞迁移至腔室，并增加腔室内IFN-γ和TNF-α水平。不同治疗组之间的细胞数量和IFN-γ水平无显著差异，但与对照动物相比，隔离应激组和冷应激组的TNF-α水平显著降低。注射CS的动物与对照组无差异。此外，应激动物在注射后第5天的TNF-α水平低于第1天，但CS组和对照组并非如此。
结果表明，在应激动物中，牙龈卟啉单胞菌在感染部位诱导的TNF-α水平下调，且CS并非唯一的介导因素。应激诱导的TNF-α水平降低可能会对经历情绪应激的人类牙周病发病机制产生影响。
