Obana A, Gohto Y, Kaneda K, Nakajima S, Takemura T, Miki T
Department of Ophthalmology, Osaka City, University Medical School, Osaka, Japan.
Lasers Surg Med. 1999;24(3):209-22. doi: 10.1002/(sici)1096-9101(1999)24:3<209::aid-lsm6>3.0.co;2-l.
To determine the optimal treatment parameters for selective occlusion of choroidal neovascularization (CNV) by photodynamic therapy (PDT) by using the photosensitizer ATX-S10 and a diode laser (wavelength = 670 nm).
Experimental CNV was induced in rat fundi by argon laser photocoagulation. The distribution of ATX-S10 in the chorioretina was analyzed by fluorescence microscopy, and the optimal treatment parameters for selective occlusion of CNV were investigated by changing the dosage and timing of laser irradiation. CNV closure and resulting damage of the surrounding tissue were documented by fluorescein angiography and light and electron microscopies.
Fluorescence of ATX-S10 was observed to be localized in the vascular lumen of the retina and choroid within 5 min after dye injection and increased in intensity in CNV up to 2-6 h and decreased rapidly in normal tissue. Laser irradiation with radiant exposures of 7.4 J/cm2 applied immediately after dye injection or with 22.0 J/cm2 at 2-4 h later effectively occluded the induced CNV without causing significant damage to normal retinal capillaries and large choroidal vessels.
PDT using ATX-S10 can selectively occlude CNV. ATX-S10 is a potentially useful photosensitizer for the treatment of CNV.
通过使用光敏剂ATX-S10和二极管激光器（波长 = 670 nm）来确定光动力疗法（PDT）选择性封闭脉络膜新生血管（CNV）的最佳治疗参数。
通过氩激光光凝在大鼠眼底诱导实验性CNV。用荧光显微镜分析ATX-S10在脉络膜视网膜中的分布，并通过改变激光照射的剂量和时间来研究选择性封闭CNV的最佳治疗参数。通过荧光素血管造影以及光镜和电镜记录CNV的封闭情况和周围组织由此产生的损伤。
在注射染料后5分钟内观察到ATX-S10的荧光定位于视网膜和脉络膜的血管腔内，在CNV中荧光强度在2 - 6小时内增加，而在正常组织中迅速降低。在注射染料后立即给予7.4 J/cm²的辐射曝光进行激光照射，或在2 - 4小时后给予22.0 J/cm²，可有效封闭诱导产生的CNV，且不会对正常视网膜毛细血管和大脉络膜血管造成显著损伤。
使用ATX-S10的PDT可选择性封闭CNV。ATX-S10是一种用于治疗CNV的潜在有用的光敏剂。
