Nagatomo I, Akasaki Y, Uchida M, Tominaga M, Kuchiiwa S, Nakagawa S, Takigawa M
Department of Neuropsychiatry, Faculty of Medicine, Kagoshima University, Japan.
Brain Res Bull. 1999 Feb;48(3):277-82. doi: 10.1016/s0361-9230(98)00171-3.
We investigated changes in numbers of nitric-oxide-producing cells in the hippocampal formation, striatum, and temporal cortex of mice 24 h after intraperitoneal administration of kainic acid (5, 10, 15, and 20 mg/kg) or domoic acid (1, 2, and 4 mg/kg). Nitric-oxide-producing cells were demonstrated histochemically by staining for nicotinamide adenine dinucleotide phosphate diaphorase. Nicotinamide adenine dinucleotide phosphate-diaphorase-positive neurons in the dentate gyrus and the subiculum did not change in number following administration of kainic acid or domoic acid at any dose. Positive neurons in the CA3 region of mice treated with kainic acid or domoic acid at any dose were significantly fewer than in controls. Although the numbers of positive neurons in the CA1/CA2 regions did not differ from those of controls at any of the four doses of kainic acid, positive cells in the CA1/CA2 were significantly more numerous than in controls at any dose of domoic acid. Although no significant differences in the numbers of positive neurons in the striatum were apparent between controls and any of the four doses of kainic acid, domoic acid significantly decreased the numbers of such cells. These results suggest that systemically administered kainic acid and domoic acid affect differentially nitric-oxide-producing cells in the hippocampal formation.
我们研究了腹腔注射海藻酸（5、10、15和20mg/kg）或软骨藻酸（1、2和4mg/kg）24小时后，小鼠海马结构、纹状体和颞叶皮质中产生一氧化氮细胞数量的变化。通过烟酰胺腺嘌呤二核苷酸磷酸黄递酶染色，组织化学法显示产生一氧化氮的细胞。给予任何剂量的海藻酸或软骨藻酸后，齿状回和下托中烟酰胺腺嘌呤二核苷酸磷酸黄递酶阳性神经元的数量没有变化。给予任何剂量海藻酸或软骨藻酸的小鼠，其CA3区的阳性神经元明显少于对照组。尽管在海藻酸的四个剂量中的任何一个剂量下，CA1/CA2区的阳性神经元数量与对照组没有差异，但在任何剂量的软骨藻酸作用下，CA1/CA2区的阳性细胞都明显多于对照组。尽管在对照组和海藻酸的四个剂量中的任何一个剂量之间，纹状体中阳性神经元的数量没有明显差异，但软骨藻酸显著减少了此类细胞的数量。这些结果表明，全身给药的海藻酸和软骨藻酸对海马结构中产生一氧化氮的细胞有不同的影响。
