Varfolomeev S D, Berezin I V
Mol Biol (Mosk). 1976 Jul-Aug;10(4):674-81.
The applications of the elements of reliability to the description of the stability and aging processes of enzymes and polyenzyme systems have been considered. The inactivation of individual enzymes or polyenzyme systems whose activity varies on the molecular level according to the "all-or-nothing" principle is described by an exponential reliability law. Systems of enzymes which are inactivated according to an exponential law have been considered. It has been shown that in the case of enzymes functioning in parallel, the value of the mean time of continuous operation is determined by the most stable enzyme, while in the case of sequentially functioning enzymes, this value is determined by the most labile. Systems whose aging process is related to the accumulation of "latent errors" and whose inactivation is characterized by gradual breakdowns are described by a normal reliability law. Systems with a combination of exponential and normal reliability laws have been considered. The use of a series of rigid quantitative criteria, such as the mean time for continuous functioning has been proposed for characterizing the stability of enzymes and polyenzyme systems. The theoretical points have been illustrated by experimental data on the stability of enzymes with hydrogenase activities, isolated chloroplasts, and cells of microscopic blue-green algae.
已经探讨了可靠性原理在描述酶和多酶系统的稳定性及老化过程中的应用。单个酶或多酶系统的失活，其活性在分子水平上根据“全或无”原则发生变化，可用指数可靠性定律来描述。已研究了按照指数定律失活的酶系统。结果表明，对于并行运作的酶，连续运行的平均时间值由最稳定的酶决定；而对于顺序运作的酶，该值则由最不稳定的酶决定。老化过程与“潜在错误”积累相关且失活表现为逐渐故障的系统，可用正态可靠性定律来描述。已考虑了具有指数和正态可靠性定律组合的系统。有人提出使用一系列严格的定量标准，如连续运作的平均时间，来表征酶和多酶系统的稳定性。关于具有氢化酶活性的酶、分离的叶绿体以及微小蓝藻细胞稳定性的实验数据，已对这些理论要点进行了说明。
