核内前体信使核糖核酸的结构。VIII. 互补序列的分离与特性分析
Structure of nuclear pre-mRNA. VIII. Isolation and characterization of complementary sequences.
作者信息
Ryskov A P, Enikolopov G N, Limborskaya S A
出版信息
Mol Biol (Mosk). 1976 Jul-Aug;10(4):748-55.
After the annealing of pre-mRNA at high Cot values, up to 20% of the material becomes resistant to the action of ribonuclease. This has been attributed to the presence of self-complementary sequences (scRNA) in the pre-mRNA. The most important properties of scRNA, which was isolated in preparative amounts, have been studied. The approximate dimensions of the complementary segments are 45-50 nucleotides. Hybridization experiments have shown that scRNA is transcribed from repeated segments of the genome. It may be postulated that the rapidly hybridizing pre-mRNA fraction consists mainly of self-complementary sequences.
在高Cot值下对前体mRNA进行退火处理后，高达20%的物质变得对核糖核酸酶的作用具有抗性。这归因于前体mRNA中存在自我互补序列（scRNA）。已经对以制备量分离得到的scRNA的最重要特性进行了研究。互补片段的大致长度为45 - 50个核苷酸。杂交实验表明，scRNA是从基因组的重复片段转录而来的。可以推测，快速杂交的前体mRNA部分主要由自我互补序列组成。
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