Tokarskaia O N, Ryskov A P
Genetika. 1982 May;18(5):713-23.
Sequence organization of two cloned mouse genomic DNA fragments which are efficiently transcribed into pre-mRNA was studied. In both cases, the arrangement of sequences hybridizing to polysomal mRNA (or cDNA), double-stranded RNA (dsRNA-B) isolated from pre-mRNA and oligo (dT), was established. In one fragment, the regions transcribed are represented by three different repetitive mRNA (or cDNA) hybridizing sequences interspersed into the AT-rich tract, the type B-2 sequence and the unique DNA sequence which also transcribed into mRNA. Nuclear pre-mRNA of 28S is hybridized to three repetitive mRNA hybridizing regions. The significance of such a complex array of repeated sequences inside eukaryotic transcription units and their possible role in mRNA processing and splicing is discussed.
对两个可高效转录为前体mRNA的克隆小鼠基因组DNA片段的序列组织进行了研究。在这两种情况下，都确定了与多聚核糖体mRNA（或cDNA）、从前体mRNA分离的双链RNA（dsRNA-B）和寡聚（dT）杂交的序列排列。在一个片段中，转录区域由三个不同的重复mRNA（或cDNA）杂交序列组成，这些序列散布在富含AT的区域、B-2型序列和也转录为mRNA的独特DNA序列中。28S的核前体mRNA与三个重复mRNA杂交区域杂交。讨论了真核转录单位内这种复杂重复序列阵列的意义及其在mRNA加工和剪接中的可能作用。
