Zeng J, Zhao L R, Nordborg C, Mattsson B, Johansson B B
Department of Neurology, Lund University Hospital, Sweden.
Brain Res Bull. 1999 Jan 15;48(2):165-71. doi: 10.1016/s0361-9230(98)00161-0.
The aim was to study if exposure to an enriched environment influenced graft-host interface and neuronal markers in neocortical grafts implanted in cortical infarct cavities 3 weeks after distal ligation of the middle cerebral artery in adult hypertensive rats. Half the rats were exposed to an enriched environment for 2 h daily 5 days a week starting 1 week after the arterial ligation. The brain was fixed by perfusion 4 weeks postgrafting. The immunoreactivity to glial fibrillary acidic protein, microtubule associated protein 2, and synaptophysin was studied in coronal paraffin-embedded sections. A distinct glial border separated the infarct cavity from the surrounding brain in sham-transplanted rats. Most grafts filled the larger part of the infarct cavity. In 8 of 18 transplants, 4 in each experimental group, part of the transplants protruded through the thin glial membrane that delineated the transplant-host interface into the adjacent host brain tissue. Microtubule associated protein 2 immunostained sections indicated bridging of dendrites in the host-transplant interface. Synaptophysin immunoreactivity was significantly higher in grafts than in contralateral cortex. However, graft morphology and neuronal marker immunoreactivity did not differ between rats housed in standard and activity stimulating cages.
目的是研究在成年高血压大鼠大脑中动脉远端结扎3周后，将新皮质移植物植入皮质梗死腔，暴露于丰富环境是否会影响移植物-宿主界面和神经元标志物。一半大鼠在动脉结扎1周后开始，每周5天，每天暴露于丰富环境2小时。移植后4周通过灌注固定大脑。在冠状石蜡包埋切片中研究了对胶质纤维酸性蛋白、微管相关蛋白2和突触素的免疫反应性。在假移植大鼠中，一个明显的胶质边界将梗死腔与周围脑组织分隔开来。大多数移植物填充了梗死腔的大部分。在18个移植体中的8个中，每个实验组4个，部分移植体穿过界定移植体-宿主界面的薄胶质膜，伸入相邻的宿主脑组织。微管相关蛋白2免疫染色切片显示宿主-移植体界面有树突桥接。移植物中突触素免疫反应性明显高于对侧皮质。然而，饲养在标准笼和活动刺激笼中的大鼠之间，移植物形态和神经元标志物免疫反应性没有差异。
