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气-水界面处的脲酶和十六烷基胺-脲酶薄膜:X射线反射和掠入射X射线衍射研究

Urease and hexadecylamine-urease films at the air-water interface: an x-ray reflection and grazing incidence x-ray diffraction study.

作者信息

Gidalevitz D, Huang Z, Rice S A

机构信息

The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.

出版信息

Biophys J. 1999 May;76(5):2797-802. doi: 10.1016/S0006-3495(99)77433-3.

Abstract

We report the results of surface x-ray scattering measurements performed on urease and hexadecylamine-urease films at the air-aqueous solution interface. It is demonstrated that although hexadecylamine does not form a stable monolayer on the pure aqueous surface, it does self-assemble into a stable, well-organized structure when spread on top of a urease film at the air-water interface. It is also likely that protein and hexadecylamine domains coexist at the interface.

摘要

我们报告了在空气-水溶液界面上对脲酶和十六烷基胺-脲酶薄膜进行表面X射线散射测量的结果。结果表明,尽管十六烷基胺在纯水表面不会形成稳定的单分子层,但当它铺展在空气-水界面的脲酶薄膜上时,会自组装成一种稳定、有序的结构。界面处蛋白质和十六烷基胺区域也可能共存。

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