Ochiai T, Honda A, Morishima T, Sata T, Sakamoto H, Satoh K
Department of Dermatology, Nihon University, School of Medicine, 30-1 Oyaguchi-Kamimachi, Itabashi-Ku, Tokyo, Japan.
Br J Dermatol. 1999 Mar;140(3):509-13. doi: 10.1046/j.1365-2133.1999.02719.x.
A woman with Hailey-Hailey disease, suffering from carcinoma of the vulva, was examined by histology and for the presence of human papillomavirus (HPV) DNA by polymerase chain reaction (PCR) and in situ hybridization. Our diagnosis by histological examination revealed the vulval carcinoma to be a squamous cell carcinoma (SCC), adjacent to lesions of Hailey-Hailey disease and severe dysplasia/carcinoma in situ [vulval intraepithelial neoplasia (VIN) III]. The PCR with consensus primers for the L1 region (L1-PCR) successfully amplified HPV DNA using total DNA extracted from formalin-fixed and paraffin-embedded tissue specimens. Restriction fragment length polymorphism analysis and sequencing of L1-PCR products revealed HPV types 16 and 39. HPV 16-specific primers for the E6 region identified HPV 16 DNA. In situ hybridization analysis with biotinylated HPV 16 and 39 DNA probes revealed the presence of the HPV 39 genome in the nuclei of the tumour cells in the SCC. These results indicate that HPV 16 and 39 are associated with lesions in vulval carcinoma. Regarding the patient's susceptibility to infection in the case of Hailey-Hailey disease, there is a possibility that HPV was inoculated into the lesions of Hailey-Hailey disease and induced those of VIN III and SCC.
一名患有黑利-黑利病的女性，同时患有外阴癌，对其进行了组织学检查，并通过聚合酶链反应（PCR）和原位杂交检测人乳头瘤病毒（HPV）DNA的存在。我们通过组织学检查诊断外阴癌为鳞状细胞癌（SCC），毗邻黑利-黑利病病变以及重度发育异常/原位癌[外阴上皮内瘤变（VIN）III级]。使用从福尔马林固定石蜡包埋组织标本中提取的总DNA，针对L1区域的通用引物进行的PCR（L1-PCR）成功扩增出HPV DNA。对L1-PCR产物进行限制性片段长度多态性分析和测序，鉴定出HPV 16型和39型。针对E6区域的HPV 16特异性引物鉴定出HPV 16 DNA。使用生物素化的HPV 16和39 DNA探针进行原位杂交分析，显示SCC肿瘤细胞核中存在HPV 39基因组。这些结果表明HPV 16和39与外阴癌病变相关。关于该患者在黑利-黑利病情况下的感染易感性，有可能HPV接种到黑利-黑利病病变中并诱发了VIN III级和SCC病变。
