Redi C A
Riv Istochim Norm Patol. 1976;20(4):177-82.
The Feulgen reaction intensity (measured with a microdensitometer Vickers M86 on the nucleus of erytrocytes of Xenopus laevis Daud.) is increased after dehistonization according to Brody (1974) only if the dehistonization is made before the fixation in acetic acid. The denaturation and renaturation treatments which should act specifically on the screws of the DNA and therefore should not affect the Feulgen reaction, act in a specific manner, probably going away another histonic components. On the dehistonized material the action of the hydrolysis of the Feulgen reaction would add up to that implicit in the dehistonization treatment and would cause a rapid fall of the values for loss of material as consequence of depolymerization facts, according to Andersson and Kjellstrand (1975). The successive renaturation treatment both on dehistonized and on non dehistonized material does not change significantly the values precedently obtained and this confirms the idea that the rilevability of the Feulgen reaction is not influenced by the treatments "per se" but by the deep "touching" of the chromatin components.
根据布罗迪（1974年）的研究，仅当在乙酸固定之前进行脱组蛋白处理时，非洲爪蟾红细胞核的福尔根反应强度（用维氏M86型显微密度计测量）在脱组蛋白后才会增加。变性和复性处理应特异性作用于DNA的螺旋结构，因此不应影响福尔根反应，但它们却以一种特定方式起作用，可能是去除了其他组蛋白成分。根据安德森和凯尔斯特兰德（1975年）的研究，在脱组蛋白的材料上，福尔根反应水解的作用会叠加到脱组蛋白处理中隐含的作用上，并由于解聚事实导致物质损失值迅速下降。对脱组蛋白和未脱组蛋白的材料进行连续复性处理，并不会显著改变之前获得的值，这证实了这样一种观点，即福尔根反应的可检测性不是受处理“本身”影响，而是受染色质成分的深度“触动”影响。
