Kurooka Y, Moriyama N, Nasu K, Kameyama S, Fukasawa R, Yano J, Kawabe K
Department of Urology, Faculty of Medicine, The University of Tokyo, Japan.
BJU Int. 1999 Feb;83(3):299-304. doi: 10.1046/j.1464-410x.1999.00904.x.
To determine the quantity and distribution of alpha1-adrenoceptor subtype mRNAs in human renal cortex.
Specimens of renal cortex tissue were obtained at the time of radical nephrectomy or total nephroureterectomy from 46 patients (mean age 59.0 years, sd 14.7) with renal cell carcinoma, renal pelvic or ureteric tumour. Using the reverse-transcriptase polymerase chain reaction (RT-PCR), the RNase protection assay and in situ hybridization, the presentation, quantity and distribution of alpha1-adrenoceptor subtype mRNAs were determined.
Expression of the three alpha1-adrenoceptor subtype mRNAs (alpha1a, alpha1b and alpha1d) was confirmed in the arteries of the renal cortex (arciform, interlobular, arteriole), but among the three subtypes, the alpha1b was less apparent by in situ hybridization. Intense alpha1-mRNA staining was apparent especially in the smooth muscle of arterial walls. In both proximal and distal renal tubules, each of the alpha1-mRNAs was less marked in cytoplasm than in the arteries. In the glomeruli weak staining was detected in the endothelium but there was no obvious staining in the veins. RT-PCR showed all three subtypes of alpha1-adrenoceptor. The RNase protection assay showed that the predominant alpha1-adrenoceptor subtype mRNA in human renal cortex was alpha1a. However, the abundance of alpha1a-mRNA in human kidney was much less than in the prostate.
Three alpha1-adrenoceptor subtype mRNAs were recognized in human renal cortex and detected particularly in the smooth muscle of the arteries. There was more alpha1a-adrenoceptor subtype in human renal cortex than the other subtypes. It is not known how each subtype operates against adrenergic stimulation; further studies are needed to examine receptor density or receptor function.
确定α1-肾上腺素能受体亚型mRNA在人肾皮质中的数量和分布。
在46例（平均年龄59.0岁，标准差14.7）肾细胞癌、肾盂或输尿管肿瘤患者行根治性肾切除术或全肾输尿管切除术时获取肾皮质组织标本。采用逆转录聚合酶链反应（RT-PCR）、核糖核酸酶保护试验和原位杂交技术，确定α1-肾上腺素能受体亚型mRNA的表达、数量和分布。
在肾皮质动脉（弓形动脉、小叶间动脉、小动脉）中证实了三种α1-肾上腺素能受体亚型mRNA（α1a、α1b和α1d）的表达，但在这三种亚型中，原位杂交显示α1b不太明显。α1-mRNA染色强烈，尤其在动脉壁平滑肌中明显。在近端和远端肾小管中，每种α1-mRNA在细胞质中的标记程度均低于动脉。在肾小球中，内皮细胞检测到弱阳性染色，但静脉中无明显染色。RT-PCR显示了α1-肾上腺素能受体的所有三种亚型。核糖核酸酶保护试验表明，人肾皮质中主要的α1-肾上腺素能受体亚型mRNA是α1a。然而，人肾中α1a-mRNA的丰度远低于前列腺。
在人肾皮质中识别出三种α1-肾上腺素能受体亚型mRNA，尤其在动脉平滑肌中检测到。人肾皮质中α1a-肾上腺素能受体亚型多于其他亚型。目前尚不清楚每种亚型如何应对肾上腺素能刺激；需要进一步研究以检查受体密度或受体功能。
