针对人疱疹病毒8型潜伏核抗原产生的单克隆抗体的特性分析
Characterization of monoclonal antibodies raised against the latent nuclear antigen of human herpesvirus 8.
作者信息
Kellam P, Bourboulia D, Dupin N, Shotton C, Fisher C, Talbot S, Boshoff C, Weiss R A
机构信息
Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB, United Kingdom.
出版信息
J Virol. 1999 Jun;73(6):5149-55. doi: 10.1128/JVI.73.6.5149-5155.1999.
Abstract
Human herpesvirus 8 (HHV-8; also designated Kaposi's sarcoma-associated herpesvirus) is the likely etiological agent of Kaposi's sarcoma (KS). HHV-8 encodes a latent nuclear antigen (LNA) which is the product of the viral gene orf 73. LNA is recognized by most infected patient sera and is the basis of current immunofluorescence assays used in epidemiological studies of HHV-8 infection. Here we describe the characterization of four monoclonal antibodies raised to the C-terminal third of LNA-glutathione S-transferase fusion proteins. These monoclonal antibodies recognized discrete linear epitopes within the C terminus and repetitive region of LNA, detected antigen in primary effusion lymphoma (PEL) cells, and precipitated a 220- to 230-kDa protein doublet corresponding to LNA from HHV-8-infected PEL cell lines. In situ immunocytochemistry of KS lesions with these antibodies show that LNA is extensively expressed in KS spindle cells.
摘要
人类疱疹病毒8型（HHV-8；也被称为卡波西肉瘤相关疱疹病毒）可能是卡波西肉瘤（KS）的病原体。HHV-8编码一种潜伏核抗原（LNA），它是病毒基因orf 73的产物。大多数受感染患者的血清都能识别LNA，它是目前用于HHV-8感染流行病学研究的免疫荧光检测的基础。在此，我们描述了针对LNA-谷胱甘肽S-转移酶融合蛋白C端三分之一区域产生的四种单克隆抗体的特性。这些单克隆抗体识别LNA C端和重复区域内的离散线性表位，在原发性渗出性淋巴瘤（PEL）细胞中检测到抗原，并沉淀出与来自HHV-8感染的PEL细胞系的LNA相对应的220至230 kDa的蛋白双峰。用这些抗体对KS病变进行原位免疫细胞化学分析表明，LNA在KS梭形细胞中广泛表达。
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