Szekely L, Chen F, Teramoto N, Ehlin-Henriksson B, Pokrovskaja K, Szeles A, Manneborg-Sandlund A, Löwbeer M, Lennette E T, Klein G
Microbiology and Tumour Biology Centre, Karolinska Institute, Stockholm, Sweden.
J Gen Virol. 1998 Jun;79 ( Pt 6):1445-52. doi: 10.1099/0022-1317-79-6-1445.
A body cavity lymphoma-derived cell line (BC1), known to carry both Epstein-Barr virus (EBV) and human herpes virus type 8 (HHV-8; or Kaposi's sarcoma-associated herpesvirus, KSHV), was analysed for the expression of EBV-encoded, growth transformation-associated antigens and cellular phenotype by immunofluorescence staining, Western blotting, RT-PCR and flow cytometry. A similar phenotypic analysis was also performed on another body cavity lymphoma line, BCBL1, that is singly infected with HHV-8. Phenotypically, the two lines were closely similar. Although both lines are known to carry rearranged immunoglobulin genes, they were mostly negative for B-cell surface markers. Both expressed the HHV-8-encoded nuclear antigen (LNA1). Similarly to Epstein-Barr nuclear antigen type 1 (EBNA1), LNA1 was associated with the chromatin in interphase nuclei and the mitotic chromosomes in metaphase. It accumulated in a few well-circumscribed nuclear bodies that did not co-localize with EBNA1. BC1 cells expressed EBNA1, LMP2A and EBV-encoded small RNAs but not EBNA2-6, LMP1 and LMP2B. They were thus similar to type I Burkitt's lymphoma cells and latently infected peripheral B-cells. Analysis of the splicing pattern of the EBNA1-encoding message by RT-PCR showed that BC1 cells used the QUK but not the YUK splice, indicating that the mRNA was initiated from Qp and not from Cp or Wp.
对一种已知同时携带爱泼斯坦-巴尔病毒（EBV）和8型人类疱疹病毒（HHV-8；即卡波西肉瘤相关疱疹病毒，KSHV）的体腔淋巴瘤衍生细胞系（BC1），通过免疫荧光染色、蛋白质印迹法、逆转录聚合酶链反应（RT-PCR）和流式细胞术分析了EBV编码的、与生长转化相关的抗原表达及细胞表型。对另一种仅感染HHV-8的体腔淋巴瘤细胞系BCBL1也进行了类似的表型分析。在表型上，这两种细胞系非常相似。尽管已知这两种细胞系都携带重排的免疫球蛋白基因，但它们大多对B细胞表面标志物呈阴性。二者均表达HHV-8编码的核抗原（LNA1）。与爱泼斯坦-巴尔核抗原1型（EBNA1）类似，LNA1在间期核中与染色质相关，在中期与有丝分裂染色体相关。它聚集在一些界限清晰的核体中，这些核体不与EBNA1共定位。BC1细胞表达EBNA1、LMP2A和EBV编码的小RNA，但不表达EBNA2-6、LMP1和LMP2B。因此，它们类似于I型伯基特淋巴瘤细胞和潜伏感染的外周B细胞。通过RT-PCR分析EBNA1编码信息的剪接模式表明，BC1细胞使用QUK剪接而非YUK剪接，这表明mRNA起始于Qp而非Cp或Wp。
