Logan T P, Millard C B, Shutz M, Schulz S M, Lee R B, Bongiovanni R
United States Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD 21010-5425, USA.
Drug Chem Toxicol. 1999 May;22(2):375-87. doi: 10.3109/01480549909017841.
The hairless guinea pig (HGP) is used by our laboratory to model the human cutaneous response to sulfur mustard (HD), bis(2-chloroethylsulfide), exposure. We determined the HD content in the skin of HGP after a 7-min exposure to vapors saturated with a mixture of HD and 14C-HD. Concentration/time (CT) values in the range of 2 micrograms/cm2/min were determined by counting skin 14C disintegrations per min (dpm) in animals euthanized immediately after exposure. These values are similar to human penetration rates obtained by other investigators. A rate curve monitoring the reduction in skin 14C dpm was developed for animals euthanized between 0 and 24 hr post- exposure. This curve showed the greatest change after 1 hr. The epidermal (62%) to dermal (38%) ratio of 14C at 24 hr was measured for two animals. We saw no site preference for HD penetration among the 8 sites used. The 14C content of template adhesive tape was determined to follow HD distribution. These results contribute to a better understanding of the cutaneous response to HD in the HGP model.
我们实验室使用无毛豚鼠（HGP）来模拟人类皮肤对硫芥（HD，双（2-氯乙基）硫醚）暴露的反应。在让HGP暴露于含有HD和14C-HD混合物的饱和蒸汽7分钟后，我们测定了其皮肤中的HD含量。通过对暴露后立即安乐死的动物皮肤每分钟14C衰变计数（dpm），确定了浓度/时间（CT）值在2微克/平方厘米/分钟的范围内。这些值与其他研究人员获得的人类渗透速率相似。为暴露后0至24小时内安乐死的动物绘制了监测皮肤14C dpm降低的速率曲线。该曲线在1小时后变化最大。对两只动物在24小时时测定了14C的表皮（62%）与真皮（38%）比例。我们发现在所使用的8个部位中，HD渗透没有部位偏好。确定模板胶带的14C含量遵循HD分布。这些结果有助于更好地理解HGP模型中皮肤对HD的反应。
