Geisenberger O, Ammendola A, Christensen B B, Molin S, Schleifer K H, Eberl L
Lehrstuhl für Mikrobiologie, Technische Universität München, Germany.
FEMS Microbiol Lett. 1999 May 1;174(1):9-17. doi: 10.1111/j.1574-6968.1999.tb13543.x.
A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4 x 10(-6) to 1 x 10(-5) transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid.
一种带有绿色荧光蛋白突变体3b（GFPmut3b）标签的广宿主范围质粒RP4衍生物，被用于监测该质粒从恶臭假单胞菌供体菌株向活性污泥中本地细菌的接合转移。测定转移频率为每受体有4×10⁻⁶至1×10⁻⁵个接合子。使用与荧光标记的、靶向rRNA的寡核苷酸进行原位杂交，以从系统发育角度确定接收了该质粒的细菌。
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