Chouroulinkov I, Lasne C
Bull Cancer. 1978;65(3):255-64.
After a short review on the two-stage (initiation and promotion) chemical carcinogenesis in vivo, the conditions required for its demonstration have been reported with several experimental data, showing the role of two distince agents, the initiator and the promotor. The same phenomenon has also been demonstrated with cell cultures in vitro. Rat embryo cells treated with an initiator and then submitted to the continuous action of a promotor have been transformed more rapidly than the untreated cells. Transformation has been tested essentially by the tumorigenicity of the cells. The results indicate that tissue culture cells are an appropriate system to investigate the mechanism of two-stage chemical carcinogenesis and allow the detection of the initiating and promoting potentialities of various compounds.
在对体内两阶段（启动和促进）化学致癌作用进行简短回顾之后，已经结合若干实验数据报道了证明该作用所需的条件，这些数据表明了两种不同试剂（引发剂和促进剂）的作用。同样的现象在体外细胞培养中也得到了证实。用引发剂处理后再受到促进剂持续作用的大鼠胚胎细胞，比未处理的细胞更快发生转化。转化主要通过细胞的致瘤性进行检测。结果表明，组织培养细胞是研究两阶段化学致癌作用机制的合适系统，并且能够检测各种化合物的启动和促进潜力。
