睾酮和5α-二氢睾酮的羟基化作用——2α-羟基睾酮的分离（作者译）
[Hydroxylation of testosterone and 5alpha-dihydrotestosterone--isolation of 2alpha-hydroxytestosterone (author's transl)].
作者信息
Wenzel M, Lemm U
出版信息
Hoppe Seylers Z Physiol Chem. 1976 Aug;357(8):1145-51.
PMID:10245
Abstract
The hydroxylation at C-2 of testosterone and 5alpha-dihydrotestosterone is measured by isolation of HTO after incubating "metabolically labile" tritium labeled steroids with the enzymes. After hydroxylation of testosterone in rat liver 2alpha-hydroxytestosterone was isolated and identified by gaschromatography and mass spectra.
摘要
通过将“代谢不稳定”的氚标记类固醇与酶一起孵育后分离出HTO，来测定睾酮和5α-二氢睾酮在C-2位的羟基化情况。在大鼠肝脏中睾酮发生羟基化反应后，通过气相色谱和质谱法分离并鉴定出了2α-羟基睾酮。
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