Wenzel M, Lemm U
Hoppe Seylers Z Physiol Chem. 1976 Aug;357(8):1145-51.
The hydroxylation at C-2 of testosterone and 5alpha-dihydrotestosterone is measured by isolation of HTO after incubating "metabolically labile" tritium labeled steroids with the enzymes. After hydroxylation of testosterone in rat liver 2alpha-hydroxytestosterone was isolated and identified by gaschromatography and mass spectra.